The Monosan® Polymer Detection Systems utilize a novel controlled polymerization technology to prepare polymeric HRP-linker antibody conjugates. Therefore, the problem of non-specific staining that can occur with Streptavidin/Biotin detection systems due to endogenous biotin does not occur.
These products are used in an immunohistochemical (IHC) procedure, which allows the qualitative identification by light microscopy of antigens in sections of formalin-fixed, paraffin-embedded tissue, via sequential steps with interposed washing steps. If required by the primary antibody, sections are subjected to epitope retrieval prior to staining. Endogenous peroxidase activity is neutralized using the Peroxidase Block (Product Code: MON-APP145).
This is followed by application of Protein Block to reduce non-specific binding of primary and polymer. The section is subsequently incubated with optimally diluted primary antibody. Monosan® Post Primary Block is used to enhance penetration of the subsequent polymer reagent. The Monosan® Polymer recognizes mouse and rabbit immunoglobulins, it detects any tissue-bound primary antibody. Sections are further incubated with the substrate/chromogen, 3,3’ - diaminobenzidine (DAB), prepared from Monosan® DAB Chromogen and Monosan® DAB Substrate Buffer (Polymer), as described below. Reaction with the peroxidase produces a visible brown precipitate at the antigen site. Sections are counterstained with Hematoxylin and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
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