Friday, 21 November 2014

2015 Calendar Giveaway


We are giving away free Wall Calendars for 2015! Ideal to pin up in your office and easily view upcoming events. If you would like one, all you have to do is email us with your full delivery address and we will send it over.

These Calendars are supplied on behalf of Cytognos - a biotechnology company committed to advancing Flow Cytometry through the design and development of new reagents, software and techniques. For more information about Cytognos and their products, please click here

Wednesday, 12 November 2014

Stem Cell – Regenerative Medicine

Caltag Medsystems are excited to bring to you a new range of Stem Cell reagents.


Stem cells offer the possibility of a renewable source of cells to replace lost function or structure for Alzheimer’s diseases, spinal cord injury, stroke, burns, heart disease, diabetes, osteoarthritis and rheumatoid arthritis. 

Cancer and birth defects occur, most often, due to abnormal cell division and differentiation of embryonic stem cells. A better understanding of the genetic and molecular regulation behind any aberrations in these processes may yield information about how such diseases arise and suggest new strategies for therapy. Moreover, new candidate drugs could be tested for safety on stem cell lines, reducing the need for animal testing. 

BioVision Stem Cell reagents are available in the UK through Caltag Medsystems. To download a copy of  BioVision's Stem Cell Research Tools, please see here. To request a hard copy brochure, please email your request to emily@caltagmedsystems.co.uk.

In addition to the new new range of Stem Cell reagents from Biovision, Caltag Medsystems supply stem cells from a number of different species; please click here for full details.
Stem Cell research can be applied to many different research purposes. Key papers referencing Biovision stem cell reagents include:

Alvero, A. B. et. al. Targeting the Mitochondria Activates Two Independent Cell Death Pathways in Ovarian Cancer Stem Cells. Mol. Cancer Ther., Aug 2011; 10: 1385 - 1393. 
Product cited:  ADP/ATP Ratio Bioluminescence Assay Kit, ApoSENSOR

Ahn et al., Human Adipose Tissue-derived Mesenchymal Stem Cells Inhibit T-Cell Lymphoma Growth In Vitro and In Vivo. Anticancer Res, Sep 2014; 34: 4839 - 4847. 
Product cited: Annexin V-FITC Apoptosis Kit

Herrmann et al., TLR9 Is Critical for Glioma Stem Cell Maintenance and Targeting. Cancer Res., Sep 2014; 74: 5218 - 5228.
Product cited: Annexin V-FITC Reagent

Leong et al., Targeting Cancer Stem Cell Plasticity Through Modulation of Epidermal Growth Factor and Insulin-Like Growth Factor Receptor Signaling in Head and Neck Squamous Cell Cancer. Stem Cells Trans Med, Sep 2014; 3: 1055 - 1065.
Product cited: Gefitinib

Abujarour et al.,Myogenic Differentiation of Muscular Dystrophy-Specific Induced Pluripotent Stem Cells for Use in Drug Discovery. Stem Cells Trans Med, Feb 2014;3:149 - 160.
Product cited: PD-0325901

Stem Cells have the potential to develop into many different cell types in the body during early life and growth. In many tissues, they serve as a sort of internal repair system, dividing essentially without limit to replenish other cells in live humans or animals. 

When a stem cell divides, each new cell has the potential either to remain a stem cell or become another cell type with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell. 


















There are many ways in which human stem cells can be used in research and the clinic. Perhaps the most important potential application of human stem cells is the generation of cells and tissues that could be used for cell-based therapies. 

If you require further information about the range of Stem Cell related products available from Caltag Medsystems please do not hesitate to contact us by emailing marketing@caltagmedsystems.co.uk or call +44 (0)1280 827460.

Cell Culture Celebration

Autumn Additions

We are offering customers purchasing any product from our Cell Culture range a free product of choice from those shown in the table below.

Latest Additions to the offer: Antibodies and Proteins for your Cell Culture Research

The offer is first come first served, so you’ll need to be fast! There’s a limited number of each free of charge product, so it’s best to check availability beforehand. Just call us on 01280 827460 and we’ll let you know. Remember to include the free of charge product on your order.

Put a zero cost next to it and we’ll know you’re not to be charged for it. Our Cell Culture range is constantly growing. We have a broad selection of Primary CellsSkinStem CellsTools and Reagents and Blood and Coagulation products to choose from.

Please contact us if you require a specific cell type, media, or human and animal tissue and we will do the sourcing for you.

Choose your free of charge product from this table, and we’ll send it out with your order:
Product code
Description
Size
Early Passage Human Coronary Artery Smooth Muscle Cells (HCASMC), cryopreserved
5x10^5 cells/vial
Early Passage Neonatal Fibroblasts
5x10^5 cells/vial
Human Umbilical Artery Endothelial Cells (HUAEC)
5x10^5 cells/vial
Human Umbilical Vein Endothelial Cells (HUVEC) - Single Donor, Cryopreserved
5x10^5 cells/vial
Human Synoviocytes
5x10^5 cells/vial
Human Epidermal Melanocytes (HEM) - Neonatal, Cryopreserved
5x10^5 cells/vial
Human Dermal Fibroblasts (HDF) - neonatal, cryopreserved
5x10^5 cells/vial
Human Dermal Microvascular Endothelial Cells (HDMEC)
5x10^5 cells/vial
C5BL/6 Mouse Lung Fibroblast
2x10^6 cells/vial
Early Passage Human Umbilical Vein Endothelial Cells (HUVEC) – pooled donor, cryopreserved
5x10^5 cells/vial
Recombinant Fibroblast Growth Factor 2, Basic (FGF2), Homo sapiens (Human)
10ug
Recombinant Interleukin 12A (IL12A), Homo sapiens (Human)
10ug
Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), Homo sapiens (Human)
10ug
Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3), Homo sapiens (Human)
100ug
Recombinant Stem Cell Factor (SCF), Mus musculus (Mouse)
10ug
Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg), Mus musculus (Mouse)
100ug
Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3), Mus musculus (Mouse)
100ug
Recombinant Stem Cell Factor (SCF), Rattus norvegicus (Rat)
10ug
Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3), Rattus norvegicus (Rat)
100ug
Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
100ug
Polyclonal Antibody to Stem Cell Factor (SCF)
100ug
Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)
100ug
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
100ug
Polyclonal Antibody to Interleukin 1 Beta (IL1b)
100ug
Polyclonal Antibody to Matrix Metalloproteinase 1 (MMP1)
100ug
Polyclonal Antibody to Connective Tissue Growth Factor (CTGF)
100ug
Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC)
100ug
Polyclonal Antibody to Interleukin 6 (IL6)
100ug
Polyclonal Antibody to Interleukin 2 (IL2)
100ug
Polyclonal Antibody to Interferon Gamma (IFNg)
100ug
Monoclonal Antibody to Interferon Gamma (IFNg)
200ug
Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)
100ug


For further information regarding our Cell Culture suppliers and products, please click here.

Friday, 7 November 2014

November Newsletter

--:Featured Products:--
Mouse Brain Vascular Pericytes (MBVP)
Catalogue #M1200
Brain Vascular Pericytes (BVP) participate in a variety of processes including angiogenesis, endothelial cell survival, regulation of capillary blood flow, and establishment and maintenance of the blood-brain barrier. Pericyte dysregulation has been linked to several pathological conditions such as hypertension, diabetic retinopathy, atherosclerosis, multiple sclerosis, Alzheimer’s disease, and tumor angiogenesis.
Bovine Plasma Vitronectin (BPV) 
Catalogue #8538

Vitronectin is involved in a number of biological functions including cell adhesion, cell spreading and migration, cell proliferation, extracellular anchoring, fibrinolysis, hemostasis, and complement immune defense. Vitronectin can be used for coating tissueculture surfaces to promote cell adhesion, proliferation, and differentiation. Optimal conditions for cell attachment must be determined for each cell line and application

Human Bronchial Epithelial Cells (HBEpiC) 
Catalogue #3210
Studies using bronchial epithelial cell culture differentiated to induce a mucociliary phenotype have shown that IL-4 and IL-13 stimulation modulate basic cell functions such as proliferation, ciliary beating, and mucous production. Cell proliferation, as well as various other cellular processes in bronchial epithelial cells, is also regulated in part by EGF receptor signaling.

Bronchial Epithelial Cell Medium (BEpiCM) 
Catalogue #3211
Bronchial Epithelial Cell Medium (BEpiCM) is a complete medium designed for normal human bronchial epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium does not contain serum.


2014 ScienCell Research Laboratories|6076 Corte Del Cedro, Carlsbad, CA 92011|(760) 602-8549
Caltag Medsystems is the UK distributor for ScienCell. For more information please contact:
T: +44(0)1280 827460 | F: +44(0)1280 827466 | E: office@caltagmedsystems.co.uk

Thursday, 6 November 2014

Chronic Aging with VitroAge: Natural aging in vitro

Traditional culture media have evolved over 50 years to provide strong proliferation for extended periods. They contain many protective and stimulatory factors and nutrients that exert a powerful anti-aging effect.

The new VitroAge medium (CnT-AG2) lacks these protective elements. Keratinocytes age naturally in this over a period of several weeks, without the need for acute doses of oxidizors or other synthetic pro-aging chemicals.

The Vitroage epidermal model maintains normal cell morphology, but generates several well documented in vivo signs of ageing including reduced lifespan and disrupted metabolism.
This unique medium is available from Caltag Medsystems, www.caltagmedsystems.co.uk.

- VitroAge Keratinocyte Aging Medium (product code: CnT-AG2), 500 ml
- VitroAge Aging Medium, Free Sample (product code: CnT-AG2-SAMPLE), 250 ml.

To request your free of charge sample, please send your order to office@caltagmedsystems.co.uk, or fax to 01280 827466. Please note that there will be a £15 shipping charge which will be needed to be included on your order.

Keratinocytes aged over 4 weeks in CnT-AG2 cycle 
progressively more slowly, and then reach senescence.
Signs of Chronic Aging In Vitro

Keratinocytes aged in the VitroAge medium for 3 weeks display a range of age-related changes also found in vivo.

Key changes include progressive loss of proliferative function, disrupted metabolism and drug oxidation.

Keratinocytes aged in VitroAge medium have also been evaluated using MRM proteomics to quantify 100 proteins in parallel.

Caltag Medsystems are the UK distrubutor for CellnTec products. For further information about their products please email office@caltagmedsystems.co.uk or call 01280 827460


Tuesday, 4 November 2014

Transfection: Magnetic Plates for Magnetofection

Magnetofection technology requires an appropriate magnetic field that magnetises nanoparticles in solution, forms a very strong gradient to attract nanoparticles onto cells and covers the surface of a culture dish.

Optimised magnetic plates with tailored properties have been specifically designed for use in Magnetofection by Oz Biosciences.

Main Features:
  • Suitable for all Magnetofection reagents and all cell culture dishes (384-, 96-, 4, 24, 12, 6-well plates, 35, 60, 90 & 100 mm dishes, T-25, T-75 and any other flasks)
  • Can be easily cleaned and decontaminated with 70% ethanol
  • Can be used within incubators and with robots
  • Can be used at room temperature, 37°C, +4°C, etc.
  • Compatible with culture plates from most common suppliers
  • Solid, completely reusable, it is a one-time buy.
3 magnetic plates are available:

Super magnetic plate                                   Dimensions: 8.3cm x12.5cm                        £315
MF10000

Magnetic plate 96-magnets                        Dimensions: 8.3cm x 12.5cm                       £288      
MF10096
Specifically adapted to 96-well culture plates

Mega magnetic plate                                     Dimensions: 20.1cmx25.7cm                      £648
MF14000
Accommodates 4 culture dishes or plates simultaneously.





Caltag Medsystems can provide the Super Magnetic Plate for a trial period of 4 weeks. Please contact office@caltagmedsystems.co.uk for further information. For details of the Magnetofection range of reagents for use with the magnetic plates, please see here.

Caltag Medsystems are the UK distributor for Oz Biosciences products. For further information about their products please click here, or email office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

The Solution for Neuron Transfection - NeuroMag

Primary neurons in culture are considered to be a highly relevant model in the study of neuronal development and activity. They can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods.

NeuroMag is a transfection reagent suitable for neurons from 1 DIV to 21 DIV. It has proven to be extremely efficient in transfecting a large variety of primary neurons such as cortical, hippocampal, dorsal root ganglion and motor neurons with all types of nucleic acids. Moreover, high transfection efficiency has also been achieved in primary astrocytes, oligodendrocyte precursors or neural stem cells as well as other cell lines (C6, B65, PC12, N2A...).

NeuroMag in November: 10% discount off all  NeuroMag products.

Features and Benefits

- Great efficiency, ideal for primary neurons.
- High transfected neurons viability.
- Efficient from 6 DIV to 21 DIV.
- Long transgene expression (up to 7 days).
- Non-toxic and completely biodegradable.
- Ready-to-use, straightforward and rapid.

NeuroMag Magnetic Transfection Reagent is available in 3 sizes:

- 1 ml     Product Code: NM51000               £552      
November Price: £495

- 500uL  Product Code: NM50500               £306
November Price: £274

- 200uL  Product Code: NM50200               £137
November Price: £123

Magnetic Plates for use with NeuroMag Magnetic Transfection Reagent

3 magnetic plates are available:

Super magnetic plate                                   Dimensions: 8.3cm x12.5cm                        £315
MF10000

Magnetic plate 96-magnets                        Dimensions: 8.3cm x 12.5cm                       £288      
MF10096 
Specifically adapted to 96-well culture plates

Mega magnetic plate                                     Dimensions: 20.1cmx25.7cm                      £648
MF14000
Accommodates 4 culture dishes or plates simultaneously

Please click here for further information about the magnetic plates. 

Applications

- Suitable for all types of nucleic acids:DNA, RNA, oligonucleotides, siRNA, mRNA, shRNA…
- Perfect for primary neurons: hippocampal, cortical, embryonic DRG, cerebellar granules, motorneurons, Neural Stem Cells...
- Highly efficient for a variety of neuronal cell lines such as A172, B65, C6, KS-1, N2A, PC12, SH-SY5Y, SKN-BE2, T98G, U251, U87, YH-13…

Recommended Application: Transfection of Primary Neurons.

References:
"High transfection efficiency on primary dopaminergic neurons at 21 DIV." Underhill SM et al, Neuron. 2014
“Achieve up to 30% of transfection efficiency on primary cortical neurons"Wang R et al, Neurobiol Dis. 2014

Please click here to view all of the references associated to the NeuroMag products.

Magnetofectin Product Range

Neuromag uses a Magnetofection technology. This requires an appropriate magnetic field that magnetizes the nanoparticles in solution, forms a very strong gradient to attract the nanoparticles onto cells and covers all the surface of the culture dish. Optimized magnetic plates with tailored properties have been specifically designed for Magnetofection, please see here

For further information about the Magnetofection technology please click here

OZ Biosciences creates, develops and produces innovative molecular delivery systems specialising in transfection and transduction tools. They focus on delivery technologies of biomolecules such as DNA, RNA and protein for in vitro and in vivo applications.


Caltag Medsystems are the UK distributor for Oz Biosciences products. For further information about Oz Bioscience products please click here, email office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

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