Monday, 22 September 2014

PCSK9- Target for the Next Blockbuster Drug

Due to the medical significance of PCSK9 (neural apoptosis-regulated convertase, NARC-1) research into its interaction with the LDL receptor is growing. To keep ahead of the demand MBL have developed a number of research reagents including PCSK9 ELISA kits, an in vitro binding assay kit and numerous antibodies.

PCSK9 inhibition:
-Lowers plasma LDL-cholesterol
-Enhances the LDL-cholesterol lowering ability of statins in patients with severe hypercholesterolemia by enhancing LDL-cholesterol lowering ability of statins.















MBL International has been leading the way in providing PCSK9 assay kits and reagents for the goal of progressing PCSK9 clinical trials.

MBL offers both the PCSK9 ELISA and the PCSK9-LDLR Bind­ing Assay – please see the table below for full product information.

Code No.
Product
Size
Price

CircuLex Human PCSK9 ELISA Kit
96 Assays
£533

CircuLex PCSK9-LDLR in vitro Binding Assay Kit
96 Assays
£540
CircuLex Mouse/Rat PCSK9 ELISA Kit
96 Assays
£492
Anti-human PCSK9
100 µg
£254
CircuLex Anti-human PCSK9 Prodomain
100 µg
£310
CircuLex Anti-human PCSK9 Prodomain
100 µg
£310
Circulex PCSK9 D374Y
10 µg
£474
CircuLex PCSK9 D374Y/δ53 in culture medium
50 µg
£237
CircuLex PCSK9 R194A (Human)
20 µg
£427
CircuLex PCSK9 Wild Type
50 µg
£427
CircuLex PCSK9 Wild Type in culture medium
50 µg
£237
Circulex PCSK9 D374Y in culture medium
50 µg
£237
Circulex PCSK9 D374Y in culture medium
50 µg
£237


Total PCSK9 Assay














The binding activity of PCSK9 and LDLR was analyzed using the CircuLex PCSK9-LDLR in vitro Binding Assay Kit. Product Code: CY-8150
As the graph shows, when PSCK9 is mutated, binding activity decreases.This kit would be ideal for looking at inhibition markers of PCSK9 activity.


Total PCSK9 Assay



Inhibitors were used to inhibit activity of PSCK9 in A431 and SW480 cells lines.  Inhibition of PCSK9 was measured using the MBL Total PCSK9 Assay, Product Code: CY-8079.

MBL is distributed exclusively in the UK by Caltag Medsystems Ltd.  View the full MBL product range here.

Contact Caltag Medsystems today to learn more about our products and how we can help you discover ways to make your science more efficient and effective: office@caltagmedsystems.co.uk

Tuesday, 9 September 2014

Transficient and siRfficient: Superior Transfection Activity against other Commercial Reagents in Numerous Adherent Cell Types

Transficient™ and siRfficient™ are transfection reagents that provide superior transfection efficiency for primary cells and a wide variety of cell lines, including those “hard-to-transfect” cell lines. They are a cost effective and highly efficient tool for your downstream applications using a simple transfection protocol.

Transficient™ : DNA Transfection Reagent
This product has been developed and optimised for delivery of nucleic acids, such as plasmid DNA and mRNA into a wide variety of cell types by creating highly stable nanoparticles that facilitates cellular uptake, endosomal escape and localization to the nucleus.

Key benefits:
  • High transfection efficiency into primary cells and a wide variety of cell lines
  • Works with or without serum
  • Easy to use
  • Robust
  • Very low toxicity.

Five different cell lines were transfected using the Luciferase gene and the TransficientTM reagent. Expression levels were compared to competitor transfection reagents.















Product Code
Product description
Pack Size
Transficient DNA Transfection Reagent
0.1ml
Transficient Transfection Reagent 0.3mL
0.3 ml
Transficient Transfection Reagent 1.0 mL
1.0 ml
Transficient Transfection Reagent, 6 x 1.0 mL
6 x 1.0 ml
Transficient Transfection Reagent, 12 x 1.0 mL
12 x 1.0 ml

Transficient DNA Transfection Reagent samples available,
please email office@caltagmedsystems.co.uk to request.

siRfficientTM : siRNA Transfection Reagent

This product has been developed and optimised for gene silencing experiments. It provides the highest efficiencies for the delivery of siRNA, microRNA or antisense RNA into the cytoplasm of a wide variety of cell types, including Neuro 2a, Jurkat, K562 and LLC-1cells.

Key benefits:
  • High transfection efficiency into primary cells and a wide variety of cell lines
  • Works with or without serum
  •  Easy to use
  • Robust
  • Very low toxicity
























A- VEGF protein levels were silenced using the siRfficient reagent, with and without serum in a Neuro 2a cell line. The results were compared Competitor silencing reagents.

B- β-actin levels were knocked down in Jurkat cells using the siRfficient reagent. Compared to Company A and Company B, siRfficient showed a 90% knockdown level

Product Code
Product Description
Pack Size
siRNA Transfection Reagent
0.3 ml
siRNA Transfection Reagent
1.0 ml
siRNA Transfection Reagent
6 x 1.0 ml
siRNA Transfection Reagent
12 x 1.0 ml

siRNA Transfection Reagent samples available, 
please email office@caltagmedsystems.co.uk to request.

If you require further information about MBL or their products please do not hesitate to contact Caltag Medsystems by emailing marketing@caltagmedsystems.co.uk or call 
+44 (0)1280 827460.

Friday, 29 August 2014

Loom Bands - How creative are you?













Loom bands  - they’ve taken the country by storm. We heard recently that a 200m long chain had been created out of loom bands in an attempt by a school in Somerset to enter the Guinness Book of Records. There’s also the loom band dress, recently auctioned on Ebay that made the news. This got us thinking…

How creative are we and how creative are Caltag Medsystems customers? For a bit of fun, we are sending out loom bands with all dispatched orders in September. 

Can you make a loom band bracelet? Or maybe a character like the one shown below? Or even a piece of clothing? We want to see all sorts of creations! Take a picture of your loom band creation and post it on our Facebook page, the best one wins a prize! We’ve posted some of our creations there too, take a look. 

 Don’t worry if you don’t know where to start– Youtube have some great tutorials: 

https://www.youtube.com/watch?v=pMzT1WY6g_g 
https://www.youtube.com/watch?v=sAaxEwl_6Y8




Monday, 18 August 2014

New Promotion: MESACUP Anti-Skin profile TEST (CE)

Limited Time Offer: 25% Discount

An Anti-Skin Profile ELISA Kit: a single assay to detect five skin specific auto-antibodies has had a massive price reduction.

We are offering 25% off the current list price, bringing the price down to £310. Simply use the code 25ABD when placing your order to take advantage of this great offer! Valid until 31 October 2014.

Autoimmune bullous diseases are a group of cutaneous disorders characterised by skin blistering or erosions and classified into pemphigus, pemphigoid, and epidermolysis bullosa aquisita (EBA).

The Anti-Skin Profile ELISA Kit can simultaneously detect five anti-Skin-specific antibodies (anti-Dsg1, anti-Dsg3, anti-BP180, anti-BP230 and anti-TypeVII collagen) in human serum, which helps in the diagnosis of patients with pemphigus (PV/PF), bullous pemphigoid (BP) and EBA.

  • Single assay detects five anti-skin-specific antibodies
  • Qualitative detection anti-Dsg1, anti-Dsg3, anti-BP180, anti-BP230 and anti-TypeVII collagen in human serum.
  • A single patient’s sample can be tested for all 5 antigens on 12 occasions
  • This is the only commercially available anti skin panel ELISA kit for diagnosis and monitoring of Autoimmune bullous diseases.

MESACUP Anti-Skin Profile ELISA Kit (CE)
Product Code: RG-7115EC-D
Price WAS: £413                NOW: £310

NB: Qualitative detection of each of anti-Dsg1, anti-Dsg3,anti-BP180, anti-BP230 and anti-TypeVII collagen in human serum can also be measured individually using single ELISAs.

BP230 ELISA (CE Mark), Product Code: RG-7613EC-D

Mesacup Desmoglein test Dsg1 (CEmark), Product Code: RG-7880EC-D

Mesacup Desmoglein test Dsg3 (CE mark), Product Code: RG-7885EC-D

BP180 ELISA Kit (CE Mark), Product Code: RG-7695-EC-D



The recombinant antigens used by this profile kit:

Skin-specific autoantibodies
Used recombinant antigens
Anti-Dsg1
Dsg1
Anti-Dsg3
Dsg3
Anti-BP180
BP180 NC16a
Anti-BP230
BP230-C and BP230-N
Anti-TypeVII Collagen
NC1 and NC2

Caltag Medsystems are the exclusive UK distributor of MBL International products. For further information about the products please visit www.caltagmedsystems.co.uk, email office@caltagmedsystems.co.uk or call +44 (0)1280 827460

Friday, 8 August 2014

Fresh and Cryopreserved Hepatocytes

Cryopreserved Hepatocytes are available from a number of species including Human, Sprague Dawley rat, CD1 mouse, Beagle dog, and Cynomolgus monkey. Each is available in a suspension grade and a monolayer grade. Human hepatocytes are also available in an induction grade. Please see the tables below for product codes.

Fresh Hepatocytes are also available – weekly inventory updates are available which contain the schedules of availability for fresh products. If you would like to sign up for email alerts, so that you are contacted when fresh hepatocytes are available, please email hannah@caltagmedsystems.co.uk.



Cryopreserved Hepatocytes: Suspension Grade

Product Code
Product Description
Male Sprague Dawley Rat cryopreserved HEPATOCYTES pooled donors, 5 x 106 cells per vial
Male CD1 mouse cryopreserved HEPATOCYTES pooled donors, 5 x 106 cells per vial
Male beagle dog cryopreserved HEPATOCYTES pooled donors, 5 x 106 cells per vial
Male cynomolgus monkey cryopreserved HEPATOCYTES pooled donors, 5 x 106 cells per vial
Human cryopreserved HEPATOCYTES, 5 x 106 cells per vial













Note – these cells are for use in suspension only, they cannot form monolayers.

Cryopreserved Hepatocytes: Monolayer Grade

Product Code
Product Description
Male Sprague Dawley Rat cryopreserved HEPATOCYTES pooled donors,  5 x 106 cells per vial
Male CD1 mouse cryopreserved HEPATOCYTES pool,  5 x 106 cells per vial
Male beagle dog cryopreserved HEPATOCYTES pool, 5 x 106 cells per vial
Male cynomolgus monkey cryopreserved  HEPATOCYTES pooled donors, 5 x 106 cells per vial
Human cryopreserved HEPATOCYTES,  5 x 106 cells per vial

Note – these cells can be seeded in culture plates and flasks and used as monolayer.

Cryopreserved Hepatocytes: Induction Grade

Product Code
Product Description
Human cryopreserved HEPATOCYTES (CYP induction grade), 5 x 106 cells per vial

Note – these cells can be seeded in culture plates and flasks and used as monolayer. The induction of several cytochromes in these cells is guaranteed.

If you require specific vial sizes and protein concentrations, bespoke vialing is also available. For further details of this or any of the hepatocyte products, please do not hesitate to contact us, either by telephone +44 (0) 1280 827460 or by email office@caltagmedsystems.co.uk.

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