Wednesday, 6 May 2015

Just a little thank you...

We know that everyone needs a break from time to time.

As a thank you to all of our customers, we are sending out packets of biscuits with all orders.
















Enjoy your coffee break with Caltag Medsystems!

Tuesday, 5 May 2015

Viro-MICST™ - Efficient and Specific Transduction

i-MICST Technology (Integrated Magnetic Immuno-Cell Sorting and Transfection/Transduction) is a new platform that allows the transduction/transfection of genetically modified cells directly on to magnetic cell purification columns. This technology combines cell isolation and genetic modification in one simple, efficient and reliable integrated system.

Ideal for sensitive cell types, such as primary and stem cells, Viro-MICST leads to an increase in the transduction efficiency with low-titer virus preparations compared to regular transduction methods.

Why use Viro-MICST?
- Allows the isolation and transduction of cells in one reliable integrated system, reducing cell manipulation steps and minimising cell stress
- Increased transduction efficiency with low MOI (multiplicity of Infection) during magnetic cell separation.
- Acceleration of the transduction process and synchronisation of adsorption
- Ideal for sensitive cell types, such as primary and stem cells
- Cells maintain their differentiation potential after using the ViroMICST procedure.

Applications:
- Recommended for the transduction/infection of cells during magnetic cell purification
- Suitable for all viruses – including AAV, Adenovirus, Lentivirus and Retrovirus
- Mammalian Cells: Adherent and suspension cells, primary and hard-to-transfect cells, cell lines and sensitive cells

Product Information

Product Code
Product Name
Number of Transductions Per Small Column*
Viro-MICST 250µl
25-50
Viro-MICST 500µl
50-100
ViroMICST 1000µL
100-200


*Based on MOI of 1 for 106 labelled-cells/column


Oz Biosciences have developed several optimised reagents for viral applications, including:
- ViroMag: For enhancing viral transduction efficiency
- ViroMag R/L: For Lentivirus and Retrovirus transduction
- AdenoMag: For Adenovirus and AAV transduction

Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

Calcium Phosphate Transfection Kit – The Ideal Method for Virus Production

The Calcium Phosphate (CaPO) Transfection Kit is perfect to transfect HEK 293 cells. This transfection method, first described by Graham and Van Der Ebb in 1973, has been optimised in order to reach higher transfection efficiency. The CaPO transfection kit is simple and easy to use. Using this kit, between 95 and 100 % of HEK 293 cells are transfected, resulting  in very high titres of virus being produced.

Applications:
- The ideal method for HEK 293 cells transfection: The Calcium Phosphate Transfection Kit is optimised for the transfection of HEK 293 cells with plasmid DNA.
- The CaPO Transfection Kit is also appropriate for a variety of immortalised cell lines, such as CHO and COS.
- It is also recommended for the production of viral vectors and proteins.

Main Features:
- Ideal for Virus Production
- High Transfection Efficiency of HEK 293 Cells
- Suitable for Producing Recombinant Proteins
- Serum Compatible
- Simple, Ready-To-Use and Rapid:
1. Plate the cells in DMEM and incubate overnight
2. Change tissue culture medium 1-2 hours before transfection
3. Prepare the DNA solution in 1x HBS
4. Add the Calcium Chloride solution, mix and incubate for 30  minutes
5. Add the complexes drop-wise to your cells

Advantages of the Calcium Phosphate Transfection Kit:
- Compaction of DNA in nanoparticles is efficiently internalised by cells
- Protection of nucleic acids against nucleases degradation
- Modified and optimised to reach higher transfection level
- Ready to use

Product Information

Product Code
Product Name
Number of Assays
CaPO Transfection Kit
100 in 100mm culture dishes with 1µg of DNA

Oz Biosciences have developed several optimised reagents for viral applications, including:
- ViroMag: For enhancing viral transduction efficiency
- ViroMag R/L: For Lentivirus and Retrovirus transduction
- AdenoMag: For Adenovirus and AAV transduction
- Viro-MICST: For the transduction/infection of cells during magnetic cell purification

Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

AdenoMag - an Adenovirus and AAV Infection Enhancer

AdenoMag is a magnetic nanoparticles-based reagent dedicated to enhance Adenovirus and Adeno-Associated Virus (AAV) infection. It allows the rapid concentration of all viral particles onto cells. AdenoMag permits to significantly improve the virus infectivity with extremely low vector doses. Due to its specific properties, AdenoMag is ideal to infect non permissive cells. No molecular biology processes or biochemical modifications are required.

Applications
- Suitable for all adenoviral and AAV vectors
- Mammalian Cells: Cell lines, primary cells and hard-to-transfect cells

Main Features:
Increases Transduction Efficiency:
-The combination of superparamagnetic nanoparticles with the adenovirus has shown up to a 500-fold enhancement of gene expression compared with standard infection

Concentrates Viral Dose:
- Improves viral infectious capacity by promoting and accelerating the infection process

Extends the Host Tropisms to Non-Permissive Cells:
- The association of viral vectors with magnetic neoparticles is sufficient to permit infection of non-permissive cells

Magnetic Targeting:
 - High transduction can be achieved under magnetic influence and confined to a specific area by the magnetic shape and position

Product Information

Product Code
Product Name
Number of Assays*
AdenoMag 100µl
500-1000 transductions in 96-well plate
AdenoMag 200µl
1000-2000 transductions in 96-well plate
AdenoMag 1mL
5000-10000 transductions in 96-well plate
AdenoMag Starting Kit
Contains 1 magnetic plate + 200µl AdenoMag


*Based on MOI of 1 for 104 Cells/well


Magnetofection™ Technology was developed by Oz Biosciences to provide a novel, simple and highly efficient transfection method across a number of applications.
The principle of Magnetofection is to associate nucleic acids, transfection reagents or viruses with specific cationic magnetic nanoparticles. The resulting molecular complexes are then concentrated and transported into cells supported by an appropriate magnetic field. In this way, the exploitation of a magnetic force exerted upon gene vectors allows a very rapid concentration of the entire applied vector dose on cells, so that 100% of the cells get into contact with a significant vector dose, promoting cellular uptake.

Oz Biosciences have developed several optimised reagents for viral applications, including:

ViroMag: For enhancing viral transduction efficiency 
- ViroMag R/L: For Lentivirus and Retrovirus transduction
- Viro-MICST: For the transduction/infection of cells during magnetic cell purification

Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

Friday, 1 May 2015

ViroMag R/L – For the Transduction of Retro and Lentiviruses

ViroMag R/L is a specific reagent optimised for retroviral and lentiviral applications.

ViroMag R/L and the virus to be transduced are mixed in a one-step procedure; no molecular biology processes or biochemical modifications are required. This reagent demonstrates an exceptionally high efficiency to promote, control and assist viral transductions.

Based on the Magnetofection™ technology, ViroMag R/L allows concentrating the complete applied dose of Retro/Lentiviral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses. Due to its specific properties, ViroMag R/L is ideal to infect non permissive cells.

Applications:
- Suitable for all retroviral and lentiviral vectors, especially VSV-G pseudo viruses
- Mammalian cells: Cell lines, primary cells and hard-to-transfect cells

Main Features:
Increases Viral Transduction Efficiency:
- Up to 100-fold gene expression enhancement
- Increases percentage of transduced cells
- Magnetic-field guided local transduction
- Transduction of a variety of cells

Improves Viral Infectious Capacity:
- Significantly enhances virus infectivity, even with very low viral doses; from 5 to 100-fold infectivity improvement

Concentrates Viral Dose:
- Increases retroviral titer from culture supernatant by 1000 to 4000 fold
- Increases virus concentration on cell surface and uptake by 70-100 fold

Promotes and Accelerates the Infection Process

Allows Synchronisation of Transduction:
- Synchronise viral cell adsorption (uptake) and accurately monitor the kinetics of the viral replication cycle

 Magnetic Targeting:
- High transduction can be achieved under magnetic influence and confined to a specific area by the magnetic shape and position

Straightforward and Non-Toxic:
- No molecular biology or biochemical processes are required

Product Information

Product Code
Product Name
Number of Assays
ViroMag R/L 100µl
30-500 transductions in 96-well plate
ViroMag R/L 200µl
60-1000 transductions in 96-well plate
ViroMag R/L 1mL
300-5000 transductions in 96-well plate
ViroMag R/L Starting Kit
Contains 1 magnetic plate + 200µl ViroMag R/L


Magnetofection™ Technology was developed by Oz Biosciences to provide a novel, simple and highly efficient transfection method across a number of applications.

The principle of Magnetofection is to associate nucleic acids, transfection reagents or viruses with specific cationic magnetic nanoparticles. The resulting molecular complexes are then concentrated and transported into cells supported by an appropriate magnetic field. In this way, the exploitation of a magnetic force exerted upon gene vectors allows a very rapid concentration of the entire applied vector dose on cells, so that 100% of the cells get into contact with a significant vector dose, promoting cellular uptake.

Oz Biosciences have developed several optimised reagents for viral applications, including:
-ViroMag: For enhancing viral transduction efficiency 
-AdenoMag: For Adenovirus and AAV transduction 
-Calcium Phosphate Transfection Kit: For HEK 293 Cells 
-Viro-MICST: For the transduction/infection of cells during magnetic cell purification

Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

ViroMag – A Viral Transduction Enhancer

ViroMag  is a versatile reagent that contains magnetic nanoparticles to increase virus infection and transduction capacities.

Based on the Magnetofection™ technology, this reagent allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus transduction with extremely low vector doses. Due to its specific properties, ViroMag is ideal to infect non permissive cells. This reagent demonstrates an exceptionally high efficiency to promote, control and assist viral transductions so that no molecular biology processes or biochemical modifications are required.

Applications
Suitable for all viral vectors:
Adenovirus, α-virus, Baculovirus, Herpes virus, lentivirus, Retrovirus, Rhabdovirus, Paramyxovirus, Polyomavirus.

Mammalian Cells:
Hard to transfect cells and cell lines, including adherent and suspension primary cells.

Main Features
Increases Viral Transduction Efficiency:
- Up to 500-fold gene expression enhancement compared to standard infection
- Suitable for in-vivo applications

Improves Viral Infectious Capacity:
- Promotes infection even with very low viral titers/doses (from 5 to 100-fold infectivity improvement)

Concentrates Viral Dose, Accelerating the Infection Process:
- Increases virus concentration from culture supernatant by 1000 to 4000 fold
- Increases viral dose concentration on cell surface and uptake by 70-100 fold
- Restores transduction efficiency of PEGylated adenovirus by association

Extends the Host Tropisms to Non-Permissive Cells:
- Association of certain viruses with ViroMag is sufficient to force infection of cells lacking a viral receptor.
- Enhances the ability to transduce in vitro target cells without modifying the virus

Allows Synchronisation of Transduction:
- Synchronise viral cell adsorption (uptake) and accurately monitor the kinetics of the viral replication cycle

Magnetic Targeting:
- High transduction can be achieved under magnetic influence and confined to a specific area by the magnetic shape and position

Product Information
Product Code
Product Name
Number of Assays
ViroMag 100µl
30-500 transductions in 96-well plate
ViroMag 200µl
60-1000 transductions in 96-well plate
ViroMag 1mL
300-5000 transductions in 96-well plate
ViroMag Starting Kit
Contains 1 magnetic plate + 200µl ViroMag


Magnetofection™ Technology was developed by Oz Biosciences to provide a novel, simple and highly efficient transfection method across a number of applications.

The principle of Magnetofection is to associate nucleic acids, transfection reagents or viruses with specific cationic magnetic nanoparticles. The resulting molecular complexes are then concentrated and transported into cells supported by an appropriate magnetic field. In this way, the exploitation of a magnetic force exerted upon gene vectors allows a very rapid concentration of the entire applied vector dose on cells, so that 100% of the cells get into contact with a significant vector dose, promoting cellular uptake.

Oz Biosciences have developed several optimised reagents for viral applications, including:
- ViroMag R/L: For Lentivirus and Retrovirus transduction 
- AdenoMag: For Adenovirus and AAV transduction 
- Calcium Phosphate Transfection Kit: For HEK 293 Cells 
- Viro-MICST: For the transduction/infection of cells during magnetic cell purification

Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.






Wednesday, 1 April 2015

Spring Savings

We're starting the Spring season by broadening our 'buy one get one free' promotion.

Yes, we know, it was already so good wasn't it! But now you can purchase any product from our website - a choice of over 200,000 products to qualify for a free product from the table below. The offer is first come first served, so you’ll need to be fast!

There's a limited number of each free of charge product, so it's best to check availability beforehand. Just call us on 01280 827460 and we'll let you know. Remember to include the free of charge product on your order. Put a zero cost next to it and we'll know you're not to be charged.

Here's some quick links to the product ranges on our website, but remember, any product purchased entitles you to a free product from the table below.

Cell Culture, Flow Cytometry, ELISA Kits, Antibodies, Molecular Biology plus many more areas to choose from.

Please contact us if you require a specific cell type, media, or human and animal tissue and we will do the sourcing for you.

Choose your free of charge product from the table below and we'll send it out with your order:

Product code
Description
Size
Cells
Early Passage Human Coronary Artery Smooth Muscle Cells (HCASMC), cryopreserved
5x10^5 cells/vial
Early Passage Neonatal Fibroblasts
5x10^5 cells/vial
Human Umbilical Artery Endothelial Cells (HUAEC)
5x10^5 cells/vial
Human Umbilical Vein Endothelial Cells (HUVEC) - Single Donor, Cryopreserved
5x10^5 cells/vial
Human Synoviocytes
5x10^5 cells/vial
Human Epidermal Melanocytes (HEM) - Neonatal, Cryopreserved
5x10^5 cells/vial
Human Dermal Fibroblasts (HDF) - neonatal, cryopreserved
5x10^5 cells/vial
Human Dermal Microvascular Endothelial Cells (HDMEC)
5x10^5 cells/vial
C5BL/6 Mouse Lung Fibroblast
2x10^6 cells/vial
Early Passage Human Umbilical Vein Endothelial Cells (HUVEC) – pooled donor, cryopreserved
5x10^5 cells/vial
Human Dermal Fibroblasts-neonate (HDF-n)
5x10^5 cells/vial
Human Brain Vascular Smooth Muscle Cells (HBVSMC)
5x10^5 cells/vial
Proteins
Recombinant Fibroblast Growth Factor 2, Basic (FGF2), Homo sapiens (Human)
10ug
Recombinant Interleukin 12A (IL12A), Homo sapiens (Human)
10ug
Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF), Homo sapiens (Human)
10ug
Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3), Homo sapiens (Human)
100ug
Recombinant Stem Cell Factor (SCF), Mus musculus (Mouse)
10ug
Recombinant Stem Cell Factor (SCF), Rattus norvegicus (Rat)
10ug
Antibodies
Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg), Mus musculus (Mouse)
100ug
Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3), Mus musculus (Mouse)
100ug
Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3), Rattus norvegicus (Rat)
100ug
Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
100ug
Polyclonal Antibody to Stem Cell Factor (SCF)
100ug
Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)
100ug
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
100ug
Polyclonal Antibody to Interleukin 1 Beta (IL1b)
100ug
Polyclonal Antibody to Matrix Metalloproteinase 1 (MMP1)
100ug
Polyclonal Antibody to Connective Tissue Growth Factor (CTGF)
100ug
Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC)
100ug
Polyclonal Antibody to Interleukin 6 (IL6)
100ug
Polyclonal Antibody to Interleukin 2 (IL2)
100ug
Polyclonal Antibody to Interferon Gamma (IFNg)
100ug
Monoclonal Antibody to Interferon Gamma (IFNg)
200ug
Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)
100ug
Antibody to Annexin A5 (ANXA5)
100ug
Antibody to Troponin I Type 3, Cardiac (TNNI3)
100ug
Antibody to Calreticulin (CRT)
100ug
Polyclonal Antibody to Collagen Type III Alpha 1 (COL3a1)
100ug
Mouse Anti-Human CD19 CE FITC
100 tests
Mouse Anti-Human CD45 TC
50 tests
Mouse Anti-Human Lysozyme FITC
50 tests
CD138 - R-PE
100 tests
ELISA Kits
BP230 ELISA Kit (CE Mark)
48 wells
Anti-TypeVII collagen ELISA Kit (CE Mark)
48 wells

Please visit www.caltagmedsystems.co.uk for further information.

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