Thursday, 30 October 2014

MHC Tetramers: Directly Measure Antigen-Specific T Cells

MHC Tetramers provide the means to rapidly detect and enumerate antigen specific T cells directly from ex vivo (patient) (donor) samples without the need for cell stimulation.  

MHC Tetramers are complexes of four Major Histocompatibility Complex (MHC) molecules associated with a specific peptide and bound to a flurochrome. The MHC/peptide complex detects antigen specific T cells at the single-cell level, surpassing detection limits of traditional cell-based methods.

Advantages of MHC Tetramers:
  • The only method to detect antigen-specific CD4+ or CD8+ T cells ex vivo
  • Reduced non-specific binding due to proprietary mutation in the alpha-3 domain in human MHC class I tetramers.
  • Immune responses are targeted to the specific allele(s)
  • Tetramer staining is quantitative
  • Does not require the use of radioactive isotopes
  • Rapid : allowing fresh blood (or tissue-derived) sample to be analysed
  • Large numbers of samples can be processed using flow cytometer
  • Tetramer-labelled cells remain viable
  • All specific CTLs are detected
  • Tetramers can be customized for unique applications

Disease Specific Tetramers:

Infectious Diseases : 
HIV-AIDS, EBV, CMV, HPV, HBV, HCV, Influenza, Malaria, TB and RSV, Chlamydia, HSV

Cancer : 
Breast, Prostate, Melanoma, Colon, Lung, Ovarian, Leukemia, Myeloma,Lymphoma, Carcinoma, Sarcoma

Autoimmune Diseases :
Diabetes, Multiple sclerosis, Autoimmune vitiligo

Transplantation :
EBV and CMV

Alleles Available:

Murine Class I Alleles
H2-Kb
H2-Kd
H2-Db
Rhesus Macaque Alleles
Muma-A*01

Please use the links below to view further details:

MHC Tetramer technology is the most accurate method for detection of rare events. The information MHC tetramers provide is pivotal to the direct development of vaccines and therapies, as well as monitoring patient responses during clinical trials. To greatly reduce non-specific binding, Class I iTAg MHC Tetramers specific to human alleles have a proprietary mutation in the a3 domain (see figure 1a and 1b below).


Further information can be found at www.mblintl.com/tetramer.

Caltag Medsystems is the UK distributor for MBL tetramers. If you are looking for a particular product, please let me know by calling +44 (0)1280 827460 or email emily@caltagmedsystems.co.uk. Full pricing details can also be found here

Human Aortic Endothelial Cells

--:Featured Products:--
Human Aortic Endothelial Cells (HAEC)
Catalogue #6100
Human Aortic Endothelial Cells (HAEC) provide an effective was to study and understand molecular mechanisms involved in many vascular alteration pathologies inaddition to the aging process. 

Recommended Medium:
It is recommended to use Endothelial Cell Medium (ECM,Cat. No.1001) for the culturing of HAEC in vitro.
View Publications>>



Catalogue #6590

Human Trabecular Meshwork Cells (HTMC) arelocated in the anterior chamber of the eye and playan active role in aqueous outflow mechanism.HTMC cultures provide an essential tool to study thefunctional control of trabecular meshwork and theopportunity to develop more efficient strategies to treat glaucoma.
Recommended Medium:
It is recommended to use Trabecular Meshwork Cell Medium (TMCM, Cat, No. 6591) for the culturing of HTMC in vitro.
View Publications>>


Mitochondrial Isolation Kit (MITOISO)
Catalogue #8268
Mitochondria Isolation Kit (MITOISO) is designed to provide optimal yeild of intact and enzymatically active mitochondria. Use MITOISO to effectively isolate mitochondria in three stages: mechanical rupturing of tissues/cells, removing cellular debris and nuclei, and harvesting mitochondria.



Human Renal Proximal Tubular Epithelial Cells
Catalogue #4100
How much time do you spend isolating your own cells? ScienCell offers an assortment of human primary cells, including renal cells, so you can focus your efforts on research in diabtes, renal fibrosis, immune diseases, drug discovery and more with ScienCell's cryopreserved human renal proximal tubular cells and renal mesangial cells.


Human Cardiac Myocytes (HCM)
Catalogue #6200

Human Cardiac Myocytes (HCM) are highly specialized high-oxygen-content cells which house an abundance of mitochondria. They are physically energetic and capable of spontaneous rhythmic depolarization and repolarization of their membranes. HCM have a complex network of signals that regulate the rhythmic pumping of the heart. A better understanding of this network will help reveal cellular mechanisms leading to cardiac myocyte death.
Recommended Medium:
It is recommended to use Cardiac Myocyte Medium (CMM, Cat. No. 6201) for the following culture of HCM in vitro.
View Publications>>


--:New To ScienCell:--
Human Thymic Epithial Cells (HTyEpiC)
Catalogue #3910
Human Thymic Epithelial Cells are an excellent in vitro model to study the mechanisms that modulate TyEpiC functionality and to develop treatments for autoimmune disorders.


Recommended Medium:
It is recommended to use Thymic Epithelial Cell Medium (TyEpiCM, Cat. #3911) for culturing FTyEpiC in vitro.



2014 ScienCell Research Laboratories|6076 Corte Del Cedro, Carlsbad, CA 92011|(760) 602-8549
Caltag Medsystems is the UK distributor for ScienCell. For more information please contact:
T: +44(0)1280 827460 | F: +44(0)1280 827466 | E: office@caltagmedsystems.co.uk

Wednesday, 29 October 2014

TF Activation Profiling Plate Arrays: Profile the Activation of up to 96 TFs

Transcription Factor (TF) Activation Profiling Arrays from Signosis are used to monitor the activities of multiple TFs simultaneously. TF Activation Profiling Plate Arrays available include:








 TF Activation Profiling Plate Arrays from Signosis allow profiling of the activation of up to 96 TFs at one time, providing a tool in facilitating the analysis of transcription factors. 8 TF Activation Profiling Plates are available. 

Benefits of Transcription Factor Activation Profiling Arrays :
  • Multiplex - One assay allows the measurement of the activities for various transcription factors.
  • Quantitative comparison Differences between samples can be quantitatively compared and analysed.
  • Simple procedures - User friendly procedures similar to ELISA.
  • No special requirement - Capital instruments such as Luminex are not needed.


TF Activation Profiling Plate Array

TF activation profiling plate arrays are used for monitoring 48 different TFs including NFkB, AP1, HIF1, and p53.

List of Applicable TFs:


TF Activation Profiling Plate Array I
Product Code FA-1001

TF Activation Profiling Plate Array II – includes two 96 well plates for the comparison of two samples.
Product Code FA-1002


Stem Cell TF Activation Profiling Plate Arrays

The Stem Cell Transcription Factor Activation Plate Array allows the analysis of up to f 16 stem cell-specific TFs simultaneously in mammalian samples. The assay can be used with the whole cell lysis from 1000-10, 000 cells.

List of Applicable TFs:




Stem Cell TF Activation Profiling Plate Array I 
Product Code FA-1003 


Cancer Stem Cell TF Activation Profiling Plate Array

Understanding the activation patterns of cancer stem cell related transcription factors in a tumour can help to understand the transcriptional mechanism of cancer stem cell and provide better approaches for targeting and eliminating cancer stem cells.

List of Applicable TFs:


Cancer Stem Cell TF Activation Profiling Plate Array 
Product Code FA-1004


Oxidative Stress TF Activation Profiling Plate Array

Oxidative stress occurs when the steady-state balance between pro-oxidant and anti-oxidant is changed, and oxidative stress has been reported to associate with many diseases, such as cancer, Alzheimer's disease, Parkinson's disease, atherosclerosis, and heart failure. Responses to oxidative stress are mediated by a large number of transcription factors (TFs).

List of Applicable TFs:


Oxidative Stress TF Activation Profiling Plate Array
Product Code FA-1005


ER Stress (UPR) TF Activation Profiling Plate Array

Early cellular response to ER stress includes the transcriptional up regulation of chaperone proteins, which is mediated by a large number of transcription factors (TFs).

List of Applicable TFs:


ER Stress (UPR) TF Activation Profiling Plate Array
Product Code FA-1006


Wnt/β-Catenin TF Activation Profiling Plate Array

The Wnt signaling pathway is important to both embryonic development and tumourigenesis.  β-Catenin, the central component of the pathway, functions as a cofactor for a number of transcription factors.  These transcription factors then activate transcription of Wnt target genes involved in cell proliferation, survival, and migration. Characterization of the activation of TF involved in the Wnt/ β-Catenin pathway can lead to greater understanding of various disease states and the development of treatments.

List of Applicable TFs:


Wnt/β-Catenin TF Activation Profiling Plate Array
Product Code
FA-1007


Cholesterol Metabolism TF Activation Profiling Plate Array

It has been widely reported that cholesterol homeostasis and metabolism are regulated by a group of transcription factors. Profiling the regulation and activation patterns of these associated transcription factors is crucial to further understanding and unravelling the intricate cholesterol metabolism pathway. 

List of Applicable TFs:


Cholesterol Metabolism TF Activation Profiling Plate Array
Product Code FA-1008


Caltag Medsystems is the UK distributor for Signosis. If you would like further information about TF Activation Profiling Plate Arrays or any other products from Signosis, please do not hesitate to contact us, either by email office@caltagmedsystems.co.uk or call us on 01280 827460.


Increase the sensitivity of flow cytometry studies with BulkLysis

BulkLysis, an isotonic lysing solution from Cytognos provides osmotic lysis of erythrocytes before immunofluorescence staining of peripheral blood or bone marrow aspirate samples.

Applications of BulkLysis

  • Increase the sensitivity of minimal residual disease detection (up to 1 cell in 100 000 cells = 0,001%). 
  • Used when nucleated cell concentration needs to be increased, such as for AML/MDS panels. 
  • Easy to apply in samples with low cell counts or to detect small/rare populations. 
  • Provided as 150 ml 10X concentrated solution containing ammonium chloride, potassium hydrogen carbonate and EDTA. This volume is sufficient for 30 tests.

To achieve greater sensitivity in immunophenotypic characterization of different cell subsets, starting with high cell numbers is recommended. This can be achieved by using BulkLysis; a fixative-free erythrocyte lysing solution that does not interfere with immunofluorescence.

The figure below shows representative flow cytometry data on normal peripheral blood treated with BulkLysis only (prior to staining), other commercial lysis with fixative agent (after staining) and the combination of the two (BulkLysis prior to staining and fixative agent lysis after staining).



Figure: Scatter characteristics of peripheral blood lysed with BulkLysis working solution and/or lysis with fixative agent (FSC threshold at 10 000; Image obtained using Infinicyt ®software, Cytognos, Spain).


Caltag Medsystems is the exclusive distributor for Cytognos in the UK and ROI. If you would like further information about BulkLysis or any other Cytognos products, please do not hesitate to contact us, either by email office@caltagmedsystems.co.uk or call us on 01280 827460.

Obesity & Diabetes Research

During obesity, excess fat accumulates in adipose tissue. Obesity is a major risk factor for many
metabolic diseases, especially diabetes and cardiovascular diseases, increasing the risk of
hypertension, hyperglycemia and dyslipidemia, recognized as the metabolic syndrome.

Caltag Medsystems supply a wide range of innovative and advanced ELISA kits and reagents in the field of ‘Obesity and Metabolism’. A selection of products available are listed below, please request a brochure for the full portfolio.

Adipokines

Adipokines are biologically active 
substances produced in white adipose
tissue (WAT) that act in an autocrine/
paracrine or endocrine fashion and 
communicate with the brain, heart,
 vasculature, liver and muscle.
  

Adipokine ELISA kits and reagents include:


Obesity-Related ELISA Kits:


Highly Active Obesity-related Transcription Factor Proteins:
FTO — A Gene Contributing to Human Obesity:

COMP-Angiopoietin-1 — Fat Droplet Size Regulation

Irisin — A New Myokine involved in Exercise & Obesity
Irisin ELISA and Irisin reagents

Interleukin-6: From Cytokine to Myokine


Fibroblast Growth Factors & Adipose Tissues
FGF-21 and FGF-19 ELISAS.


Fibroblast growth factors (FGFs) are signalling proteins with diverse functions in development, 
metabolism and neural function.


Zinc-a2-glycoprotein — Marker of Fat Catabolism

Betatrophin — Linking Adipose Tissue & β Cells

NEW “Browning” Agents

AMPK — A Metabolic Master Switch 

AdipoRon - An AdipoR Agonist











Netrin-1 – Macrophage Retention in Adipose Tissue


Streptozotocin – Standard Diabetes Inducer


Adipogen also supply ELISA kits for research in the areas of Cancer, Immunology and Inflammation. To View the full range of Adipogen products, click here. If you would like to request a full brochure of Adipogen’s products or you cannot find what you are looking for in our full ELISA list please email.

If you have any questions, please do not hesitate to contact us by email tara@caltagmedsystems.co.uk or by telephone +44 (0) 1280 827460.

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