Wednesday, 29 June 2016

DNA Methylation Research Just Got Easier

DNA Methylation Research Just Got Easier
Caltag Medsystems
Accurately Measure Global 5-mC or 5-hmC in DNA in Just 2 Hours!
Now you can save yourself the extra effort and quantify global 5-mC or 5-hmC levels in DNA in just one easy step with Epigentek’s MethylFlash™ Global DNA Methylation and Hydroxymethylation ELISA Easy Kits. These two new kits allow you to quantify global DNAmethylation or hydroxymethylation status by specifically measuring levels of 5-mC or 5-hmC in a simplified, "one-step" ELISA-like format. 
Advantages & Features of MethylFlash Easy Kits:
  • Fast - Reduced steps allow you to finish in only 2 hours
  • Robust - Improved kits have a greater “signal window” with reduced variation between replicates
  • Convenient - Inherently low background noise without the need for DNA denaturation and plate blocking steps
  • Sensitive - Detection limit can be as low as 0.05% methylated or 0.01% hydroxymethylated DNA from 100 ng of input
  • DNA Specific - No cross-reactivity to unmethylated or other modified cytosines
  • Universal - Included positive and negative controls allow for detection of DNA methylation or hydroxymethylation in any species from either single-stranded or double-stranded input
  • DNA Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assays to be more accurate and highly comparable with HPLC-MS analysis
  • Flexible - Strip-well microplate format for manual or high throughput analysis
Plus, save 25% for a limited time! 
How are these new kits different from the previous MethylFlash kits?
The MethylFlash Easy kits, while still retaining the many advantageous features of the previous kits, utilises a “one-step” ELISA reaction to eliminate the need for multiple sample and antibody binding steps. The protocols of these new kits are simplified, reducing the procedure times by 50% versus the previous MethylFlash kits. Some adjustments were made to also reduce the CV% between wells. The greater “signal window” of each kit, along with adjusted calculation formulas, makes these new kits very accurate. In a nutshell, MethylFlash Easy kits are faster, easier, more robust, and more accurate.
Epigentek products are distributed in the UK by Caltag Medsystems Ltd.

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