Friday, 28 August 2015

Quantify Foetal Red Blood Cells: Introducing the FMH QuikQuant Assay

The enumeration of foetal red blood cells (fRBCs) is used to calculate the extent of Foetomaternal Haemmorhage (FMH), either in the case of trauma with suspected placental injury, or in the situation of RhD incompatibility between the foetus and the mother. The amount of fRBCs is a measure for the prevention of haemolytic disease of the newborn using prophylactic anti-D therapy.

The most widely used assay for FMH detection has been the visual microscopic counting Kleihauer Betke (KB) method, which is based upon differences in solubility properties in acid conditions of fetal hemoglobin (HbF) from adult hemoglobin. While the KB method is easily performed by most clinical laboratories, it lacks sensitivity and exhibits poor reproducibility or precision (CVs of 50-100%). 

The FMH QuikQuant assay uses an anti-Haemoglobin F monoclonal antibody and Propidium Iodide as a specific marker for nucleated cells. The whole assay takes less than one hour to complete, making it more efficient than the KB assay – as well as more sensitive and precise. Whilst the KB assay is performed by most clinical laboratories, the method exhibits poor reducibility or precision (CVs of 50-100%). Using FMH QuikQuant, samples with as low as 0.04% fRBCs can be significantly (P<0.05) distinguished from samples lacking foetal cells, as shown in the table below:

Table 1. Determination of assay sensitivity was performed by dilution studies of Trillium FETALtrol mixtures (high, low and negative levels) followed by replicate measurements on a Beckton Dickenson FACScan instrument.

Key Features:
- CE/IVD for FMH quantification
- Detection of HbF as low as 0.06% foetal cells in maternal blood
- Results within 1 hour with two single centrifuge steps

Product Information:
FMH QuikQuant (CE/IVD)
Product Code: QQF-100

Related Products:
FETALtrol (CE/IVD); A haematologic control for the detection of foetal red blood cells in maternal circulation.
- Available in 2 Sizes: 1 x 2ml and 2 x 2ml.

Caltag Medsystems are the exclusive UK and Ireland distributor for Trillium Diagnostics. For further information about the FMH QuikQuant or any of their products, please call +44 (0)1280 827460 or email

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