Wednesday, 8 July 2015

Detect Autoantibodies Relating to Bullous Pemphigoid

The MESACUP BP180 and MESACUP BP230 ELISA Kits from MBL provide a semi-quantitative ELISA for the detection of anti-BP180 and anti-BP230 antibodies in human serum using recombinant immobilised antigens. The kits can be used independently (84% and 58% clinical significance respectively) or in conjunction with another for higher clinical significance (97%) and can be used for the differential diagnosis and monitoring of Bullous Pemphigoid activity (1).


Key Features


  • Highly Specific: differential diagnosis of Bullous Pemphigoid from other autoimmune blistering diseases.
  • Monitor disease activity through autoantibody levels
  • High Sensitivity: combine kits for higher significance
  • Economical breakaway strips

MESACUP BP180 ELISA Kit (48 well)

Anti-BP180 Ig is thought to be the pathogenic antibody, with 84% (54/64) of patients testing positive in the active stage of the disease (2, 3).

  • Price: £205 (excluding VAT)
  • Product code: RG-7695-EC-D

MESACUP BP230 ELISA Kit (48 well)

Not all BP patients have the anti-BP180 antibody in their serum. Anti-BP230 Ig is also highly specific to BP and considered to be a useful serologic marker of the disease, with 58% (37/64) of patients testing positive in the active stage of the disease (1, 4). A clinical significance of 97% can be achieved using both kits (1).

  • Price: £205 (excluding VAT)
  • Product code: RG-7613EC-D

Monitoring disease activity in BP with the BP180 ELISA kit

Several clinical studies suggested that the data using the BP180 ELISA kit demonstrated that the disappearance of blisters correlated with decrease in the amount of anti-BP180 antibody (1, 5, 6). These findings strongly suggest the clinical importance of periodic measurements of the autoantibodies in patients with BP.


References:


  1. Yoshida M, Hamada T, Amagai M, Hashimoto K, Uehara R, Yamaguchi K, Imamura K, Okamoto E, Yasumoto S, Hashimoto T. Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid. J. Dermatol. Sci. 41: 21-30, 2006, PubMed: 16364599
  2. Leighty L, Li N, Diaz LA, Liu Z. Experimental models for the autoimmune and inflammatory blistering disease, Bullous pemphigoid. Arch. Dermatol. Res. 299: 417–22, 2007, PubMed: 17879094
  3. Kobayashi M, Amagai M, Kuroda-Kinoshita K, Hashimoto T, Shirakata Y, Hashimoto K, Nishikawa T. BP180 ELISA using bacterial recombinant NC16a protein as a diagnostic and monitoring tool for bullous pemphigoid. J. Dermatol. Sci. 30: 224-32, 2006, PubMed: 12443845
  4. Kasperkiewicz M, Zillikens D. The pathophysiology of bullous pemphigoid. Clin. Rev. Allergy Immunol. 33: 67–77, 2007, PubMed: 18094948
  5. Lee EH, Kim YH, Kim S, Kim SE, Kim SC. Usefulness of Enzyme-linked Immunosorbent Assay Using Recombinant BP180 and BP230 for Serodiagnosis and Monitoring Disease Activity of Bullous Pemphigoid. Ann. Dermatol. 24: 45-55, 2012, PubMed: 22363155
  6. Patsatsi A, Kyriakou A, Pavlitou-Tsiontsi A, Giannakou A, Sotiriadis D. Association of autoantibodies to BP180 with disease activity in Greek patients with bullous pemphigoid. Clin. Dev. Immunol. 2012: 854795, 2012, PubMed: 23227089

Alternative Autoimmune Blistering Kits include:

MESACUP Anti-Type VII collagen
MESACUP-2 Desmoglein Test Dsg1
MESACUP-2 Desmoglein Test Dsg3
Anti-Skin Profile Test

4 comments:

  1. nice article… simple and useful :)

    TOSHIBA PVM-375AT

    ReplyDelete
  2. While medications are accessible to treat bullous pemphigoid, a variety of Pemphigoid Natural Treatment can also be extremely powerful.

    ReplyDelete
  3. Bullous pemphigoid is a rare, chronic condition happening for the most part in elderly people. The Bullous Pemphigoid Natural Treatment includes use of antibiotics, ointments and anti-inflammatory medicines.

    http://www.herbs-solutions-by-nature.com/Bullous-Pemphigoid.php

    ReplyDelete
  4. Patients should use natural and herbal remedies for Bullous Pemphigoid Herbal Treatment . these remedies work successfully without any side effects.

    ReplyDelete

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