Thursday, 23 July 2015

Fresh Hepatocytes

Freshly isolated hepatocytes are widely accepted as a superior in-vitro model for evaluating the in-vitro hepatic metabolism and toxicity of test compounds because they more closely reflect the in-vivo situation, when compared with other models.

Biopredic International has a well-organised supply network which guarantees the regular delivery of human liver resects and animal livers. Human liver is obtained from partial hepatectomies and from whole livers rejected for transplantation. High quality fresh human and animal hepatocytes are isolated from the livers and then fully characterized.

Fresh human hepatocytes products include:

- Hepatocyte suspensions suitable for short-term metabolism studies (up to 4 hours)

- Plated (monolayer) hepatocytes cultures for longer-term metabolism studies (up to 3 days).

Hepatocytes suspensions suitable for short-term metabolism studies (up to 4 hours)

In order to provide suspended human hepatocytes, freshly isolated hepatocytes from human liver tissue are rapidly suspended and preserved in Biopredic’s optimized hypothermic medium to maintain cell viability and functional activity during shipment  (up to 48hours).

Key features of human fresh hepatocytes in suspension

- Good availability of fresh cells with proprietary production process
- High quality product combined with optimized hypothermic shipping condition
- Stable and retained viability and expression level of metabolizing enzymes 
- Greater flexibility to handle cells either in suspension or plated cell culture
- Characterization data available for each product batch sheet 
- General patient information (age, gender, race, health conditions, serology)
- Characterization for fresh human hepatocytes in suspension

Biopredic provides the following data for each lot of hepatocyte suspension:

- Viability after isolation and after cold shipping period, yield, plateability after cold storage period, morphology before and after shipment
- Phase I drug-metabolizing enzymes with a cocktail of probe substrates: CYP1A2/ 3A4/ 2B6/ 2D6/ 2C19
- Phase II drug-metabolizing enzymes: UGT1A1/ UGT1A6/ UGT1A9/ UGT2B15/ SULT1A1/ SULT1A3/ 4/ SULT1E1
- Serological and sterility control data

Shipment of fresh human hepatocytes in suspension

Freshly isolated hepatocytes are suspended in an optimized hypothermic medium and then aliquoted into cryovials containing at least 10 million cells per vial (1ml).The cell suspension is shipped the same day of the preparation (or the following day) at hypothermic condition (2-8°C).

The viability, plateability and functionality of preserved cell suspension are well maintained for at least 48h at 4°C.

Fresh Hepatocytes Email Alert Service

To receive dates of intended surgeries and to reserve hepatocytes, please sign up here.

To view the available fresh suspension hepatocytes products, please click here.

Plated (monolayer) hepatocytes cultures for longer-term metabolism studies (up to 3 days)

To provide adherent monolayer cultures, freshly isolated and viable cells are plated at a desired seeding density onto collagen-coated culture supports according to the customer’s request/ordering.

After overnight attachment, the seeding medium is replaced with either a short-term culture medium or long-term culture medium (Lanford et al., 1989, journal and vol, page?) to produce short-term (≤ 3 days) or long-term hepatocytes (≤ 6 days) cultures in which cells retain a differentiated phenotype for at least 1 week.

Both freshly prepared short-term and long-term cultured hepatocytes contain all major phase I and phase II enzymes as well as the whole gene regulation pathway necessary to investigate the potentiality of drugs to induce phase I and phase II enzymes.

Key features of human fresh plated hepatocytes

- Multi-format plated cells and custom preparation available
- Greater viability and confluence of cells in monolayer
- High expression levels of major hepatic drug-metabolizing enzymes, co-factors and cell membrane receptors
- Greater flexibility for applications with either short or long-term cultures
- Optimized packaging and shipping conditions with Biopredic's patented technology - SEALOVAC
- Full characterization data available for each product batch sheet
- General patient information (age, gender, race, health conditions, serology)
- Characterization of human fresh plated hepatocytes

Biopredic International provides the following data for each lot of plated hepatocytes:

- Cell viability and cell culture period after isolation and seeding
- Cell morphology and culture confluence by microphotography
- Phase I drug-metabolizing enzymes with a cocktail of probe substrates: CYP1A2/ 3A4/ 2B6/ 2D6/ 2C19)
- Phase II drug-metabolizing enzymes: UGT1A1/ UGT1A6/  UGT1A9/ UGT2B15/ SULT1A1/ SULT1A3/ 4/ SULT1E1
- Serological and sterility control data

Shipment of human fresh plated hepatocytes

Plated hepatocytes cultured into multi-well plates are firstly filled with either short-term or long-term culture medium (with or without Phenol red) according to customer’s specification/request.

The pre-filled plate is then packaged using Biopredic's patent-pending Sealovac™ Technology for the shipment at room temperature (18-30°C).
Cell quality and functional activity of packaged monolayer cells are well maintained for at least 48hours.

Fresh Hepatocytes Email Alert Service

To receive dates of intended surgeries and to reserve hepatocytes, please sign up here.

To view the available fresh monolayer hepatocytes products, please click here.

Fresh animal hepatocytes

Biopredic International provides a wide range of fresh animal hepatocytes obtained from a variety of species: rat, mouse, hamster, rabbit, cynomolgus monkey, dog, minipig and other species (available on demand).

Fresh animal hepatocytes can be prepared from either single animal liver donor (such as mouse, rat, dog, monkey, rabbit or hamster) or pooled from several animal donors (mouse, rat or rabbit) according to our production schedule.

All rodent hepatocytes are isolated from animals housed in our animal facility which is managed in strict compliance with the Directive 2010/63/EU of the European Parliament and of the Council.

Hepatocytes of other animal species are isolated from the liver tissues collected following approved protocols and respecting all ethical and legal requirements.

Fresh animal hepatocytes products include:
- Hepatocytes suspensions
To view the available fresh suspension hepatocytes products, please click here.

- Plated (monolayer) hepatocytes cultures
To view the available fresh monolayer hepatocytes products, please click here.

Fresh Animal Hepatocytes Email Alert Service

To receive alerts when fresh animal hepatocytes are available, please sign up here.


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