Thursday, 23 July 2015

Cryopreserved Hepatocytes

Biopredic International has more than 20 years of in-house experience in cryopreserving human and animal hepatocytes.

Cryopreserved hepatocytes are offered in two formats. These are suitable for either suspension applications or plateable applications. 

Cryopreserved Human Hepatocytes for Suspension Applications

All suspension qualified human cryopreserved hepatocytes have post-thaw viabilities over 85%.  They are also characterized for P450 and Phase II drug-metabolizing enzyme activity.  Both single donor and pooled human cryopreserved hepatocytes are suitable for use in a variety of applications including metabolic stability, clearance, metabolite ID, metabolite profiling, species comparison studies, and uptake transporter studies.

Single Donor Cryopreserved Human Hepatocytes, Suspension Qualified are produced from a single human donor.  Each lot number represents a specific individual.  Demographic information can be used as a factor in the selection of specific lots of these hepatocytes.

Pooled Cryopreserved Human Hepatocytes, Suspension Qualified are a source of pooled hepatocytes, and are produced from multiple, single donors to represent an average population phenotype.  Note that pooled human cryopreserved hepatocytes should be thawed with pooled human cryopreserved hepatocyte medium for the optimum results.

Please contact Caltag Medsystems to request the current inventory or for a quotation. Email office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

Cryopreserved Human Hepatocytes for Plated Applications

Plateable hepatocytes are offered in different grades according to the application required
- Cryoplateable Human Hepatocytes (Grade P)
- Cryoplateable and inducible Human Hepatocytes (Grade I)
- Cryoplateable & sandwich culture hepatocytes

All plateable cryopreserved human hepatocytes have post-thaw viabilities over 85%.  Each lot can also be plated in a collagen-coated plate or similar.  These lots are guaranteed to maintain a healthy monolayer for a minimum of 3, 5, or 7 days in culture, depending on their qualification.  The monolayer confluency must be over 85% for the duration of the culture to be qualified as a plateable lot.

Cryoplateable Human Hepatocytes (Grade P)

Cryopreserved Human Hepatocytes, Plateable, Metabolism Qualified, can be used for multiple applications, including the study of low-clearance compound metabolism.  Each lot is tested for metabolic activity of known low-clearance substrates for CYP2C9, CYP2D6, and CYP3A4.  These lots are guaranteed plateable for a minimum of 3 days in culture.

Cryoplateable and inducible Human Hepatocytes (Grade I)

Cryopreserved Human Hepatocytes, Plateable, Induction Qualified, can be used for a variety of applications; including to determine if compounds of interest are inducers of CYP1A2, CYP2B6, and/or CYP3A4.  Qualification includes testing induction of each isoform with a known inducer and measuring the fold change in mRNA expression and activity.  These lots are guaranteed plateable for a minimum of 5 days in culture.

Cryoplateable & sandwich culture hepatocytes

Cryopreserved Human Hepatocytes, Plateable, Transporter Certified can be used for multiple applications including uptake and efflux transporter assays such as facilitated active uptake and B-Clear.  These lots are characterised and demonstrate transporter uptake and efflux activity similar to fresh human hepatocytes.  Qualyst Transporter Solutions (Durham, NC) characterises these cells as experts in the hepatic transporter field.  These lots are guaranteed plateable for a minimum of 7 days in culture.

Please contact Caltag Medsystems to request the current inventory or for a quotation. Email office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

Cryopreserved Animal Hepatocytes

Triangle Research Laboratories provides cryopreserved hepatocytes from a variety of clinical species, including, but not limited to:

- Cryopreserved Mouse – 24 hours
- Rat (Sprague-Dawley, Wistar Han, Wistar)
- Mouse (CD-1 and C57Bl/6)
- Dog (Beagle)
- Monkey (Cynomolgus)

Every lot of animal cryopreserved hepatocytes is characterised for viability/stability, attachment efficiency, monolayer confluency, and general Phase I and Phase II drug metabolizing enzyme activities.  There is a wide selection of lots that can be used for a variety of applications, including, but not limited to:

- Drug metabolizing enzyme activity
- Suspension drug transporter uptake
- Time-dependent metabolism (plated)
- CYP450 Induction
- Plated drug transporter assays
- Toxicity assays

Cryopreserved Animal Hepatocytes for Suspension Applications

All suspension qualified animal cryopreserved hepatocytes have post-thaw viabilities over 80%.  These lots are also characterized for general Phase I and Phase II drug-metabolizing enzyme activity.

Please contact Caltag Medsystems to request the current inventory or for a quotation. Email office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

Cryopreserved Animal Hepatocytes for Plated Applications

All plateable cryopreserved animal hepatocytes have post-thaw viabilities over 80%.  Each lot can also be plated in a collagen-coated plate or similar.  These lots are guaranteed to maintain a healthy monolayer for a minimum of 3 days in culture.  The monolayer confluency must be over 85% for the duration of the culture to be qualified as a plateable lot.  These lots are also characterized for general Phase I and Phase II drug-metabolizing enzyme activity.

Please contact Caltag Medsystems to request the current inventory or for a quotation. Email office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

For instructions on how to handle cryopreserved hepatocytes, including thawing and plating:
Thawing and Plating Cryopreserved Hepatocytes (2303)

For instructions to count hepatocytes with the Trypan Blue Exclusion Method:
Cell Counting with Trypan Blue Protocol (6666)


Caltag Medsystems are the exclusive UK and Ireland distributor for Biopredic products. For further information about hepatocytes or any of their other products please call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

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