Wednesday, 17 June 2015

TransFix - A Cellular Antigen Stabilisation Reagent

What is TransFix?

TransFix is a stabilisation solution that stabilises cellular suspensions of both human and animal origin whilst preserving the integrity and antigenicity of leukocytes for transportation and storage. The components of TransFix stabilise cellular markers, allowing analysis by flow cytometry to be carried out at a more convenient time for the user. TransFix can be used in clinical diagnostic testing, clinical trials and a wide range of research applications…

Human Blood Stabilisation
For immunophenotyping, untreated blood samples have a viable shelf life of 24-48 hours due to enzymatic degradation of cellular markers. Treating blood samples with TransFix enables flow cytometric analysis of extracellular markers at 2°C to 25°C for 10 days and at 37°C for 3 days.

TransFix is available in several formats including TransFix Sample Storage Tubes and TransFix/EDTA Vacuum Blood Collection Tubes (direct draw collection tubes used to stabilise venous blood at the point of collection and preserve whole blood specimens for immunophenotyping by flow cytometry). TransFix is also available in bulk volumes.


CSF Stabilisation
TransFix/EDTA CSF Sample Storage Tubes have been developed in collaboration with research oncologists and contain TransFix that has been optimised for CSF leukocyte stabilisation.

Ordinarily, CSF samples require flow cytometric analysis within 2 hours of lumbar puncture, as the immunophenotypic profile is detrimentally affected by natural degradation. The components of the TransFix reagent used in TransFix/EDTA CSF Sample Storage Tubes stabilise CSF leukocyte markers, making it possible to determine cell subsets by flow cytometric analysis up to 10 days after collection.

A recent paper in the British Journal of Haematology (Guidelines on the use of multicolour flow cytometry in the diagnosis of haematological neoplasms, Johansson et al, 2014) recommended that unless immediate analysis is possible then CSF samples can be stored in TransFix/EDTA CSF Sample Storage Tubes for up to 72 hours before analysis.


 Bone Marrow Stabilisation
TransFix has been shown to stabilise cells within human bone marrow populations. This allows immunophenotyping by flow cytometry to be conducted at a time that is more convenient to the operator.

Independent research involving the use of TransFix to stabilise bone marrow samples has been published, including the use of TransFix for the enumeration of bone marrow mast cells  and for immunophenotyping rat bone marrow stem cells

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Immunostaining of Circulating Tumour Cells
The presence of circulating tumour cells (CTC’s) has been shown to be of diagnostic relevance for patients with early and advanced breast cancer. The usefulness of CTC assessments depends upon accurate cell counts and the corresponding analysis of molecular targets.
TransFix stabilised blood samples were used for CTC analysis in an independent study. CTCs were detected by image analytics after fluorescence scanning microscopy.


Stabilisation of Animal Blood
Researchers have found that the addition of TransFix to animal blood samples at the time of collection has significantly extended the integrity of the samples in several different species.
Independent research studies involving the use of TransFix in stabilising animal blood samples have been published:
- A rapid high precision flow cytometry based technique for total white blood cell counting in chickens
- Effects of Cyclosporin A induced T-lymphocyte depletion on the course of avian Metapneumovirus (aMPV) infection in turkeys. 
TransFix has also been used to successfully stabilise whole blood from the following species: mouse, rat, guinea pig, sheep, seal, turtle, ostrich and pig. However, these studies have yet to be published.

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TransFix is produced by Cytomark - a division of Caltag Medsystems. If you would like further information about TransFix, please do not hesitate to contact us at, or telephone (+44) 01280 827460

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