Monday, 8 June 2015

Functional Antibodies From Adipogen

Antibodies displaying an agonist or antagonist activity (functional grade antibodies (FuncAbs™)) are powerful tools for mimicking or blocking physiological functions in vitro and in vivo.

The mechanism of action for functional antibodies varies depending on its role:

Blocking: Antibodies can bind directly to ligands, preventing them from activating their cognate receptors or binding directly to their cognate receptors and inhibit receptor activation or function.  A potent blocking antibody is anti-Angiopoietin-2 mAb (rec.), able to inhibit the binding of mouse and human angiopoietin-2 to its receptor Tie-2.

Activation/Induction: Binding to receptors can also induce active signals that alter cellular fates. A potent activating antibody is anti-CD40 (mouse), mAb (FGK45), able to activate B and NK cells in vivo and in vitro.

Depletion: Binding of cell surface receptors can result in depletion of antigen-bearing cells through complement-mediated lysis and opsonisation, as well as Fc receptor for IgG (FcγR)-mediated clearance. A potent neutralizing antibody is anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14), able to deplete 98% neutrophils in vivo (250 μg/mouse).

Functional grade antibodies from AdipoGen Life Sciences are available free of preservatives and tested for low endotoxin content and may be used for activation, neutralizing or blocking studies, both in vitro or in vivo.

To view the brochure of Adipogen's functional antibodies, please click here. Alternatively, if you would like us to send you a physical copy please contact us at marketing@caltagmedsystems.co.uk.

Antibodies Available:

Blocking/Neutralising Antibodies: 

anti-APRIL (mouse), mAb (rec.) (blocking) (Apry-1-1)
- Inhibits binding of mouse APRIL to mouse BCMA and TACI

anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4) 
- Inhibits the binding of human angiopoietin-2 to human Tie-2 (Human IgG2λ)

anti-Angiopoietin-2 mAb (rec.) (blocking) (Angy-2-1)
- Inhibits the binding of mouse/human angiopoietin-2 to human Tie-2 (Mouse IgG2bλ)

anti-IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1)
- Inhibits the binding of mouse IL-33 to ST2/IL-1RAcP

anti-BAFF (human), mAb (blocking) (4.62)
- Inhibition/Neutralising of human BAFF binding to Raji cells

anti-TRAIL-R1 (human), mAb (HS101)
- Inhibition/Neutralising (blocks TRAIL-R1 mediated killing if applied in solution)

anti-TRAIL-R2 (human), mAb (HS201)
- Inhibition/Neutralising (blocks TRAIL-R2 mediated killing if applied in solution)

anti-NS5B (HCV), mAb (blocking) (5B-12B7)
- Blocks the RNA-dependent RNA polymerase activity in vitro

anti-BTLA (human), mAb (6F4)
- Inhibits interaction of BTLA to HVEM or UL144

anti-LAG-3, mAb (blocking) (11E3)
- Blocks LAG-3/MHC class II interactions

anti-LAG-3 (human), mAb (blocking) (17B4)
- Blocks LAG-3/MHC class II interactions

anti-VEGF-A (human), mAb (3(6D3))
- Inhibits VEGF-A signaling

Inducing/Activating Antibodies:

anti-LTβR (mouse), mAb (4H8 WH2)
- Agonists inducing BAFF, chemokines and integrins in vitro and in vivo (Rat IgG2a)
- Also suitable for FACS

anti-LTβR (mouse), mAb (3C8)
- Agonists inducing BAFF, chemokines and integrins in vitro and in vivo (Rat IgG1κ)

anti-CD40 (mouse), mAb (FGK45)
- Activates B and NK cells in vivo and in vitro

anti-Fas (human), mAb (APO-1-3)
- Induces apoptosis with or without cross-linking (Protein A), depending on cell type

Depleting Antibodies:

anti-BAFF-R (mouse), mAb (9B9)
- Depletes B cells in vivo

anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14)
- Optimal reagent to deplete neutrophils in vivo (250 μg/mouse)

Conformation-specific Recombinant Antibodies:

anti-Rab1-GTP, mAb (rec.) (ROF7)
- Recognises human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP

anti-Rab6-GTP, mAb (rec.) (AA2)
- Recognises human, mouse and drosophila GTP-bound
Rab6a and Rab6b and mutant Rab6Q72L. Does not detect
Rab6-GDP

anti-Tubulin-GTP, mAb (rec.) (MB11)
- Recognises human, mouse, rat and drosophila tubulin-GTP

Other Recombinant Monoclonal Antibodies:

anti-Giantin, mAb (rec.) (TA10)
- Recognises human and mouse giantin

anti-HMGB1, mAb (rec.) (Giby-1-4)
- Recognises human, mouse and rat HMGB1

anti-IL-1R2 (mouse), mAb (rec.) (Praxy-1-1)
- Recognises mouse IL-1R2

anti-Myosin IIA (non-muscle) (heavy chain), mAb (rec.) (SF9) 
- Recognises human, mouse, rat and drosophila myosin
IIA (heavy chain)

anti-α-Tubulin, mAb (rec.) (F2C)
- Recognises mouse, bovine and human α-tubulin

anti-β-Tubulin, mAb (rec.) (S11B)
- Recognises human, mouse, rat, pig, drosophila and
monkey β-tubulin

anti-IL-33 (mouse), mAb (rec.) (Carly-1-4)
- Recognises mouse IL-33

anti-PEDF (human), mAb (rec.) (Serpy-1-4)
- Recognises human PEDF

anti-EGFP, mAb (rec.) (G3)
- Recognises EGFP, ECFP and EYFP



Post-translational Modification-specific Antibody for
Cancer Research:


anti-Polyglutamylation Modification, mAb (GT335)
- Recognises most forms of polyglutamylated tubulin (α- and β-tubulin), independent of the length of the glutamate side chains. No specificity to particular tubulin isoforms nor to tubulin from particular species are observed. Detects also other (poly)glutamylated proteins. Since no consensus modification site is known for protein (poly)glutamylation, the detection is not sequence-specific. However, an acidic environment of the modification site is required.

Inflammasome Signalling Blocking Antibody:

anti-Asc, pAb (AL177)
- Inhibits interaction between Asc and NLRP3, leading to blockade of caspase-1 processing in vitro.

Caltag Medsystems are the UK distributor for Adipogen Life Sciences. For more information about any of our products, please contact us at office@caltagmedsystems.co.uk or call (+44) 01280 827460.

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