Monday, 8 June 2015

Chromatin Immunoprecipitation Kits

In the past, chromatin immunoprecipitation assays involved lengthy, labour-intensive procedures, often resulting in a low throughput and less enrichment efficiency. Epigentek’s EpiQuick and ChromaFlash ranges of ChIP kits mark a vast improvement in performance, efficiency, and convenience, providing an invaluable tool for studying in vivo protein-DNA interaction. The most popular Epigentek products are detailed in the table below:




Purpose
Selective immuno-precipitation and enrichment of chromatin fractions from low amount of cells or tissues
Selective immuno-precipitation and enrichment of chromatin fractions from cells
Selective immuno-precipitation and enrichment of chromatin fractions from tissues
Selective immuno-precipitation and enrichment of chromatin fractions from plants
Immuno-precipitate and shear isolated chromatin using magnetic beads in one step
Key Points
Recommended for next-gen sequencing applications
Robust protocol designed specifically for cell input
Robust protocol designed specifically for tissue input
Robust protocol designed specifically for plant tissue input
Ultra-fast protocol designed specifically for isolated chromatin input
Starting Material
Cells or Tissues
Cells
Tissues
Plant Tissues (flowers, leaves, young seedlings)
Isolated Chromatin
Input Range
2,000 to 1,000,000 cells or 0.5 to 50 mg tissue per reaction
1,000,000 to 2,000,000 cells per reaction
10 to 30 mg tissue per reaction
20 to 50 mg tissue per reaction
5 to 10 µg chromatin per reaction
Total Protocol Time
5 hours
5 hours
5 hours
6 hours
1 hour (with EpiSonic) or 4 hours (without EpiSonic)
Relative-Fold Enrichment vs IgG
500+
100 to 200
100 to 200
100 to 200
100 to 200
*All products include negative controls, DNA purification columns, and control primers where required*


ChromaFlash ChIP Kits

Epigentek’s ChromaFlash CHiP kits provide fast, cost efficient immunoprecipitation of specific chromatin fragments. The microplate format allows flexibility, allowing single reactions or high throughput assays, and all products are compatible with various downstream analysis workflows including ChIP-PCR, ChIP-on-chip, and ChIP-seq.

The ChromaFlash High Sensitivity ChIP Kit allows for selective immunoprecipitation and enrichment of chromatin fractions from a low amount of cells or tissues whilst reducing background noise.
Product code: P-2027-24 (24 reactions)| Price: £216.00
Product code: P-2027-48 (48 reactions)| Price: £347.00

The ChromaFlash One-Step Magnetic ChIP Kit is used to immunoprecipitate and shear isolated chromatin using magnetic beads in one step. The ultra-fast protocol is designed specifically for isolated chromatin input.
Product code: P-2026-48 (48 reactions)| Price: £276.00
Product code: P-2026-96 (96 reactions)| Price: £465.00

EpiQuik ChIP Kits

Epigentek’s EpiQuik range provides a rugged procedure for selective immunoprecipitation and enrichment of chromatin fractions from a variety of starting materials. The convenient strip microplate format allows for manual or high throughput assays.

EpiQuik ChIP Kit for chromatin immunoprecipitation from cell input.
Product code: P-2002-1 (24 reactions)| Price: £198.00
Product code: P-2002-2 (48 reactions)| Price: £321.00
Product code: P-2002-3 (96 reactions)| Price: £486.00

EpiQuik Tissue ChIP Kit for chromatin immunoprecipitation from tissue input.
Product code: P-2003-1 (24 reactions)| Price: £198.00
Product code: P-2003-2 (48 reactions)| Price: £321.00
Product code: P-2003-3 (96 reactions)| Price: £486.00

EpiQuik Plant ChIP Kit for chromatin immunoprecipitation from plant tissue input.
Product code: P-2014-24 (24 reactions)| Price: £260.00
Product code: P-2014-48 (48 reactions)| Price: £425.00

Also introducing…

EpiNext ChIP-Seq High-Sensitivity Kit (Illumina)

The EpiNext ChIP-Seq High Sensitivity Kit is designed to streamline the process of ChIP sequencing, combining selective enrichment of a chromatin fraction containing specific DNA sequences and preparation of a ChIP-Seq library for next generation sequencing.
  • Capture of low abundance protein/DNA complexes with minimized non-specific background levels.
  • Construct both non-barcoded (singleplexed) and barcoded (multiplexed) ChIPSeq libraries quickly with reduced bias for use with Illumina sequencing platforms.
Product code: P-2030-12 (12 reactions)| Price: £459
Product code: P-2030-24 (24 reactions)| Price: £858


Caltag Medsystems are the UK distributor for Epigentek. If you would like further information about their ChIP kits or any of our products please contact us at office@caltagmedsystems.co.uk or telephone 01280827460

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