Tuesday, 5 May 2015

Calcium Phosphate Transfection Kit – The Ideal Method for Virus Production

The Calcium Phosphate (CaPO) Transfection Kit is perfect to transfect HEK 293 cells. This transfection method, first described by Graham and Van Der Ebb in 1973, has been optimised in order to reach higher transfection efficiency. The CaPO transfection kit is simple and easy to use. Using this kit, between 95 and 100 % of HEK 293 cells are transfected, resulting  in very high titres of virus being produced.

Applications:
- The ideal method for HEK 293 cells transfection: The Calcium Phosphate Transfection Kit is optimised for the transfection of HEK 293 cells with plasmid DNA.
- The CaPO Transfection Kit is also appropriate for a variety of immortalised cell lines, such as CHO and COS.
- It is also recommended for the production of viral vectors and proteins.

Main Features:
- Ideal for Virus Production
- High Transfection Efficiency of HEK 293 Cells
- Suitable for Producing Recombinant Proteins
- Serum Compatible
- Simple, Ready-To-Use and Rapid:
1. Plate the cells in DMEM and incubate overnight
2. Change tissue culture medium 1-2 hours before transfection
3. Prepare the DNA solution in 1x HBS
4. Add the Calcium Chloride solution, mix and incubate for 30  minutes
5. Add the complexes drop-wise to your cells

Advantages of the Calcium Phosphate Transfection Kit:
- Compaction of DNA in nanoparticles is efficiently internalised by cells
- Protection of nucleic acids against nucleases degradation
- Modified and optimised to reach higher transfection level
- Ready to use

Product Information

Product Code
Product Name
Number of Assays
CaPO Transfection Kit
100 in 100mm culture dishes with 1µg of DNA

Oz Biosciences have developed several optimised reagents for viral applications, including:
- ViroMag: For enhancing viral transduction efficiency
- ViroMag R/L: For Lentivirus and Retrovirus transduction
- AdenoMag: For Adenovirus and AAV transduction
- Viro-MICST: For the transduction/infection of cells during magnetic cell purification

Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

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