Tuesday, 5 May 2015

AdenoMag - an Adenovirus and AAV Infection Enhancer

AdenoMag is a magnetic nanoparticles-based reagent dedicated to enhance Adenovirus and Adeno-Associated Virus (AAV) infection. It allows the rapid concentration of all viral particles onto cells. AdenoMag permits to significantly improve the virus infectivity with extremely low vector doses. Due to its specific properties, AdenoMag is ideal to infect non permissive cells. No molecular biology processes or biochemical modifications are required.

Applications
- Suitable for all adenoviral and AAV vectors
- Mammalian Cells: Cell lines, primary cells and hard-to-transfect cells

Main Features:
Increases Transduction Efficiency:
-The combination of superparamagnetic nanoparticles with the adenovirus has shown up to a 500-fold enhancement of gene expression compared with standard infection

Concentrates Viral Dose:
- Improves viral infectious capacity by promoting and accelerating the infection process

Extends the Host Tropisms to Non-Permissive Cells:
- The association of viral vectors with magnetic neoparticles is sufficient to permit infection of non-permissive cells

Magnetic Targeting:
 - High transduction can be achieved under magnetic influence and confined to a specific area by the magnetic shape and position

Product Information

Product Code
Product Name
Number of Assays*
AdenoMag 100µl
500-1000 transductions in 96-well plate
AdenoMag 200µl
1000-2000 transductions in 96-well plate
AdenoMag 1mL
5000-10000 transductions in 96-well plate
AdenoMag Starting Kit
Contains 1 magnetic plate + 200µl AdenoMag


*Based on MOI of 1 for 104 Cells/well


Magnetofection™ Technology was developed by Oz Biosciences to provide a novel, simple and highly efficient transfection method across a number of applications.
The principle of Magnetofection is to associate nucleic acids, transfection reagents or viruses with specific cationic magnetic nanoparticles. The resulting molecular complexes are then concentrated and transported into cells supported by an appropriate magnetic field. In this way, the exploitation of a magnetic force exerted upon gene vectors allows a very rapid concentration of the entire applied vector dose on cells, so that 100% of the cells get into contact with a significant vector dose, promoting cellular uptake.

Oz Biosciences have developed several optimised reagents for viral applications, including:

ViroMag: For enhancing viral transduction efficiency 
- ViroMag R/L: For Lentivirus and Retrovirus transduction
- Viro-MICST: For the transduction/infection of cells during magnetic cell purification

Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

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