Wednesday, 18 February 2015

Reporter Gene Stabilised mRNA

Reporter Gene mRNAs

Reporter genes are commonly used in cell biology research. Reporter gene mRNA can be used as controls to study transfection and expression in mammalian cells using a variety of assays. The capped (Cap 0) and polyadenylated mRNAs is optimised for mammalian systems and modified to reduce immune stimulation. They mimic fully processed mature mRNA.

 mRNA transfection provides several advantages :

             -No need for nuclear uptake - protein expression occurs directly in cytoplasm
     -Faster protein expression than DNA transfection
     -No genomic integration
     -Protein expression directly related to mRNA quantity
     - Perfect for transfecting slow or non-dividing cells
     - Protein expression in a total promoter-independent manner
     -Transient transfection: mRNA based expression of proteins is sustained for a limited time

GFP mRNA has been designed to express high levels of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. 













LUC mRNA
has been designed to express high levels of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is photostable and resistant to photobleaching. 

β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyses the conversion of β-galactosides into monosaccharides. It is a common marker gene used to assess transfection efficiency.

Product Code
Description
Size
mRNA GFP
20 µg
mRNA GFP
100µg
mRNA Luciferase
20µg
mRNA Luciferase
100µg
mCherry mRNA
20µg
mCherry mRNA
100µg
Beta Gal mRNA
20µg
Beta Gal mRNA
100µg



Caltag Medsystems are the UK distributor for OZ Biosciences. For further information about their products, please visit our website, www.caltagmedsystems.co.uk, call +44 (0)1280 827460 or email office@caltagmedsystems.co.uk.

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