Tuesday, 4 November 2014

Magnetofection: a novel, simple and highly efficient transfection method.

Unique technology suitable for viral and non-viral gene delivery applications.

In Vitro Magnetofection: Principle
The principle of Magnetofection™ is to associate nucleic acids, transfection reagents or virus with specific cationic magnetic nanoparticles. The resulting molecular complexes are then concentrated and transported into cells supported by an appropriate magnetic field. In this way, the exploitation of a magnetic force exerted upon gene vectors allows a very rapid concentration of the entire applied vector dose on cells, so that 100% of the cells get in contact with a significant vector dose, and promotes cellular uptake. Magnetofection can be used both in vitro and in vivo

In Vitro Magnetofection: What are the applications?

Magnetofection™ is the only versatile and universal technology adapted to all types of nucleic acids (DNA, siRNA, dsRNA, shRNA, mRNA, ODN...), non-viral transfection systems (transfection reagents) and viruses. Several optimized reagents have been designed according to defined applications:

- PolyMag / PolyMag Neo: for all nucleic acids transfection
- NeuroMag: for neurons transfection
- CombiMag:for enhancing all transfection reagents efficiency (cf Magnetofectamine)
- SilenceMag: for siRNA applications
- ViroMag: for enhancing viral transduction efficiency
- AdenoMag: for Adenovirus and AAV transduction
- FluoMag: Fluorescent Magnetofection Reagents
- SelfMag: for creating your own magnetic delivery system

Do I need specific equipment?

The only requirement for Magnetofection™ is a magnetic plate specifically designed for this application.

Please click here to view the different magnetic plates that are available.

Please click here for further information about the magnetic plate. 

In Vivo Magnetofection™: Principle

The unique solution for in vivo targeted gene delivery.

The main problems currently associated with systemic gene vector administration (gene therapy) include bio distribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo milieu and other side effects due to high vector doses. Magnetofection™ resolves the problems related to diffusion limited process and to restricted bioavailability at the target site.

In vivo Magnetofection™ has been designed for in vivo targeted transfection and infection. This original system combines magnetic nanoparticles and nucleic acid vectors that will be retained after injection at the magnetically targeted site. In this way, targeted delivery minimizes systemic distribution and reduces toxicity.

Furthermore, the magnetic force will enhance the uptake of magnetic nanoparticles by the target tissue, and thus improve the efficiency of transfection/transduction. This allows reducing the required nucleic acid or virus doses and the process time of delivery which is crucial for improvement of in vivo nucleic acid delivery.

In Vivo Magnetofection™: How do I use the reagents?

Gene vectors /nanoparticles complexes can be easily administrated through various injection routes such as:

-Systemic administration (intravenous, intra-artery) or local administration (intratumoral, intracerebroventricular, intraperitoneal, intramuscular, subcutaneous).

The only requirement for In vivo Magnetofection™ is a small magnet specifically designed for this application. Several kinds of magnets are provided depending of your application, please see here.

Caltag Medsystems are the UK distributor for Oz Biosciences products. For further information about Oz Bioscience products please click here, email office@caltagmedsystems.co.uk or call +44 (0)1280 827460.

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