Friday, 13 June 2014

E2 Screening Kits

Protein ubiquitination requires coordinated actions of Ub activating enzymes (E1), Ub conjugating enzymes (E2), and Ub ligase (E3). Human cells have two E1s, approximately 40 E2s and more than 800 E3s. Some E2 enzymes catalyze formation of specific polyubiquitin linkages, thus conferring polyUb chain linkage specificity in ubiquitination reactions.

UBPBio’s E2 screening kit contains 26 active E2 enzymes, Ub activating enzyme (E1), Ub, ATP and reaction buffer. Customisation of your own E2 screening kit is available by selecting ten E2s.

The UBPBio  E2 screening kit was recently used in identification of the UbE2D family of E2s in mediating in situ ubiquitination of the 26S proteasome, which was found to downregulate proteasomal activity (Jacobson AD, et al. Autoregulation of the 26S proteasome by in situ ubiquitination. Mol Biol Cell. 2014, April 17. [Epub ahead of print]
 
UBPBio provide more than 45 Products for Your Study of Ub/Ubl Conjugation

E1 Enzymes
UbE1; SAE1/SAE2; APPBP1/UBA3

E2 Enzymes
Ube2A (Rad6A)
Ube2B
Ube2C (UbcH10)
Ube2D1 (UbcH5A)
Ube2D2 (UbcH5B)
Ube2D3 (UbcH5C)
Ube2D4 (UbcH5D)
Ube2E1 (UbcH6)
Ube2E2 (UbcH8)
Ube2E3 (UbcH9)
Ube2F
Ube2G1
Ube2G2
Ube2H (UbcH2)
Ube2I
Ube2K (E2-25K)
Ube2L3
Ube2L6
Ube2M (UbcH12)
Ube2N (Ubc13/Uev1a)
Ube2Q2
Ube2R1 (UbcH3, Cdc34)
Ube2R2
Ube2S
Ube2T
Ube2V2
Ube2W



UBPBio Products are distributed in the UK by Caltag Medsystems Ltd. Please Call + 44 (0) 1280 827460 or email office@caltagmedsystems.co.uk for further information.


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