Thursday, 15 May 2014

Analyse the Angiogenic Pathway



www.cellworks.co.uk
Analyse the Angiogenic Pathway

Cellworks offers cells, kits and reagents for in vitro co-culture angiogenesis assays including:

- The V2a Kit™ - Vasculogenesis to Angiogenesis
        - A ready to use, human co-cultured in-vitro                   angiogenesis assay kit. 
- Proven Angiogenesis Tested Cells and Media for           Bespoke Assay Design
        - Brings further assay flexibility
        - Allows cell transfection prior to cell culture
- Where do you read the latest journals?



The V2a Kit™ - Vasculogenesis to Angiogenesis

“An in vitro human model of angiogenesis that includes all the known phases of the angiogenic process”

The V2a Kit contains all of the cells and reagents necessary to successfully complete a 24 well angiogenesis assay and includes a cryogenically preserved ampoule of matched cells, 24 well tissue culture plate, medium, supplements, control compounds and all staining reagents.

- Designed to allow testing of compounds, media or tissue explants
- 24 well format
- Test compounds can be added at any time (from the onset of vasculogenesis through to advanced angiogenesis)
- Analyse any aspect of the angiogenic pathway (cell activation, cell proliferation, migration, elongation and anastomosis) with complete flexibility of experimental timing.

Positive and negative controls are included in the V2a Kit.
 Tubule formation in response                 Untreated tubules                   Tubule formation in response
     to VEGF (enhancer)                                                                            to suramin (inhibitor)

Molecules shown to be active with the V2a Kit
InhibitorsEnhancers                          
Antibody to VEGFVEGF
VEGF Antisense OligonucelotidePlatelet Derived Growth Factor
SuraminBasic Fibroblast Growth Factor
CurcuminTumour Cell conditioned Medium
TGFb-1
Matrix Metalloproteinase Inhibitor
Thalidomide Analogues
For further information regarding the V2a Kit (Product Code ZHA-4000) please click here.

Cellworks provide the cells, media and supporting reagents for bespoke angiogenesis assays
Our angiogenesis tested cells allow flexibility in designing angiogenesis assays. They bring complete control over the number and cell types present in the assay, and the opportunity to manipulate the cells prior to culturing, for example by transfection.
Human Umbilical Vein Endothelial Cells (HUVEC) - pooled donor - Angiogenesis Tested
Product Code: ZHC-2102
  • Produce tubules in conjunction with Cellworks Angiogenesis Tested Human Dermal Fibroblasts
  • Validated for use in the manufacture of Cellworks V2a Kits
  • Minimum of 500,000 cryopreserved cells
  • Guaranteed for 10 population doublings
  • Cultured using Cellworks Angiogenesis Seeding and Growth Medium (ZHA-1960 and ZHA-1970, respectively)
Human Dermal Fibroblasts (HDF) - adult - Angiogenesis Tested
Product Code: ZHC-5102
  • Produce tubules in conjunction with Cellworks Angiogenesis Tested Human Umbilical Vein Endothelial Cells
  • Validated for use in the manufacture of Cellworks V2a Kits
  • Minimum of 1,000,000 cryopreserved cells
  • Guaranteed for 10 population doublings
  • Cultured using Cellworks Angiogenesis Seeding and Growth Medium (ZHA-1960 and ZHA-1970, respectively)
Early Passage Human Umbilical Vein Endothelial Cells (HUVEC) - pooled donor
Product Code: ZHC-2301
  • Cryopreserved at the end of the secondary culture
  • Not angiogenesis tested, but a cost effective alternative to buying primary cells which require additional passaging
  • Minimum of 500,000 cryopreserved cells from multiple donors
  • Guaranteed for 10 population doublings
Supporting Reagents:
CD31Tubule Staining Kit – Product Code: ZHA-1225
Angiogenesis Assay Control Kit – Product Code: ZHA-1300
Media and Supplements:
Angiogenesis Growth Medium Package. Product Code: ZHA-1970
Angiogenesis Seeding Medium Package. Product Code: ZHA-1960
Human Large Vessel Endothelial Cell Growth Medium Package. Product Code: ZHM-2953
Human Fibroblast Growth Medium Package. Product Code: ZHM-5923

For further information regarding Cellworks products please click here.

Where do you read the latest journals?
Here at Cellworks we would love to know which publications you read and a little bit about your research too. It helps ensure we are developing products that our customers have highlighted as being important to them. Below is a link to a short survey that we would gratefully appreciate your feedback in. As a thank you for helping us out, we are offering 5x £50/$85/€60 amazon vouchers to the five customers supplying the most helpful sets of answers. 
Please access the questions by clicking here

Cellworks is a division of Caltag Medsystems dedicated to the manufacture of angiogenesis reagents. To order; simply call, fax or email using the following contact details:
Tel: +44 (0) 1280 827460 or 0800 279 9113
Fax: +44 (0) 1280 827466 or 0800 279 9114
Email: cellworks@caltagmedsystems.co.uk
Address: Whiteleaf Business Centre, 11 Little Balmer, Buckingham, MK18 1TF, UK. 
www.cellworks.co.uk <http://www.cellworks.co.uk> 

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