Friday, 4 October 2013

Autophagy: Meet Some More of our Key Reagents

I hope you had a chance to read our previous blog: Autophagy, an Extensive Line up of Autophagic Targets in which we detailed a number of our best performing LC3 reagents suitable for use in multiple applications. 

This time, we’d like to highlight some other Key Reagents in the Autophagy Portfolio that are: 
- For Use in Multiple Applications 
- Validated and Widely Referenced 
- Cost Effective
 - Antibodies raised to p62, Beclin 1, Atg5 and Atg16L. 

 “p62 links ubiquitin-proteasome system to autophagic process.” 

For Multiple Applications: Anti-p62/SQSTM1 polyclonal antibody, Product Code PM045 is the de facto standard for detecting p62/SQSTM1. Many publications are available containing data using this antibody. Suitable applications include: WB, IP, ICC, IHC, FC. Reactivity: Human, Mouse, Rat.



Anti-p62/SQSTM1 polyclonal antibody, Product Code PM045 in immunocytochemistry. p62 aggregates have been found in cancer and neurologic disorders. 

“Beclin 1 is involved in autophagosome formation.” 

Anti-Beclin 1 polyclonal antibody, Product Code PD017; has been verified in Dr. Yoshimori’s laboratory. Multiple applications include WB, IP, ICC, IHC-Fr. Mutations of the Beclin-1 gene are found in human ovarian, breast and prostate cancer. 














Anti-Beclin 1 polyclonal antibody, Product Code PD017, following immunoprecipitation of HA tagged Beclin1. Lane 1: IP with normal rabbit IgG, Lane 2: IP with anti-HA-tag (M132-3), Lane 3: IP with PD017. 

“Atg5 forms complex with Atg12 and Atg16L, and this complex is required for autophagosome formation.” 

Anti-Atg5 polyclonal antibody, Product Code PM050, detects endogenous Atg5 in WB with high specificity. Reactivity: Human, Mouse, Rat. Click here to view citations. 










Anti-Atg5 polyclonal antibody, Product Code PM050 in WB. Lane 1: Atg5-/- MEF, Lane 2: MEF, Lane 3: NIH/3T3, Lane 4: HeLa, Lane 5: 293, Lane 6: NRK, Lane 7: PC12. 

"Atg16L forms a complex with Atg5 and Atg12, and this complex is required for autophagosome formation.” 

Anti-Atg16L polyclonal antibody, Product Code PM040 is suitable for use in WB, IP, ICC.















Anti-Atg16L polyclonal antibody, Product Code PM040, used in Immunoprecipitation. 

The full autophagy reagent line up can be viewed here, please contact us if you can’t find what you are looking for as new reagents are released constantly. 

Caltag Medsystems is the exclusive UK distributor for MBL Products. Please email (office@caltagmedsystems.co.uk) or phone (01280 827460) if you require further information.

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