Monday, 22 April 2013

B Cell Activating Factor

Are you interested in a new highly active, human B cell-activating factor (BAFF) that activates BAFF-R, TACI & BCMA Receptors?

BAFF, Soluble (human) (60-mer) (rec.) (highly active)
Product Code
: AG-40B-0112-C010  Pack Size: 10 µg
Product Code: AG-40B-0112-3010  Pack Size: 3 x 10 µg

Source:  E. Coli
Sequence: The extracellular domain of human BAFF (aa 134-285) is fused at the N-terminus to a His-tag.
Specificity: Binds to human and mouse IL-2R
Biological Activity: Increases CD23 expression on B cells in vivo. 

BAFF (B cell-activating factor; BLyS; B lymphocyte stimulator; TNFSF13B; TALL-1; CD257), belonging to theTNF family, is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. BAFF co-stimulates activated T cells. Increased levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome, rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (SLE). 
Furthermore, BAFF is found in inflammatory sites in which there is lymphoid neogenesis. BAFF levels are elevated in patients with multiple myeloma and B cell chronic lymphoid leukemia (B-CCL).

Processed human BAFF can either remain as a trimer or assemble into 60-mer composed of 20 trimers. Despite the predominant functional role of processed BAFF in vivo, membrane-bound BAFF might also play a role. Indeed, soluble BAFF (3-mer) can trigger BAFF-R but not TACI or BCMA, whereas oligomeric forms of BAFF (BAFF 60-mer), which mimic membrane-bound BAFF, activate all BAFF receptors.

References: TACI, unlike BAFF-R, is solely activated by oligomeric BAFF and APRIL to support survival of activated B cells and plasmablasts: C. Bossen, et al.; Blood 111, 1004 (2008).
Mutation of the BAFF furin cleavage site impairs B-cell homeostasis and antibody responses: C. Bossen, et al.; Eur. J. Immunol. 41, 787 (2011)

Figure: BAFF, Soluble (human) (60-mer) (AG-40B-0112) binds and activates BCMA receptor.

These products are available from Caltag Medsystems, the UK distributor for Adipogen. For our complete range of BAFF reagents please take a look here.

If you have any questions, please do not hesitate to contact me by email or by telephone +44 (0) 1280 827460.

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