Tuesday, 22 January 2013

BriClone Now Distributed by Caltag Medsystems

Caltag Medsystems Ltd are pleased to announce that they are now distributing BriClone Hybridoma Cloning Medium.

BriClone is a conditioned medium collected from a human cell line, supplied frozen in 100ml aliquots. It is a cloning medium suitable for use in the post fusion stages of hybridoma production and for cloning hybridoma cells under conditions of limiting dilution. When added to culture media, BriClone replaces the function of feeder cells.

BriClone Hybridoma Cloning and Selection Supplement (25ml) 
Product Code: ZKBRICLONESAMPLE

BriClone Hybridoma Cloning and Selection Supplement (100ml) 
Product Code: ZKBRICLONE

Why use BriClone?
The use of feeder cells to support the outgrowth of hybridomas has several disadvantages. These include the potential problem of batch to batch variation in the quality of feeder cells, plus feeder cells compete with freshly fused hybridomas resulting in the depletion of vital nutrients from the culture medium. BriClone has been developed to overcome these common problems that occur with feeder cells.

Benefits:
  1. Increased efficiency: BriClone is more efficient then feeder cells in cloning by limiting dilution methods
  2. Reduces risk of culture contamination
  3. Eliminates batch variation
  4. No depletion of nutrients by feeder cells
  5. No time consuming preparations
  6. Mycoplasma free

An example of hybridoma cloning using BriClone (5%) or mouse macrophages as feeder cells. The hybridomas were cloned by limiting dilution at 1 cell/well.

Call the Caltag Medsystems office now on +44 (0)1280 827 460 or email office@caltagmedsystems.co.uk  to order your Briclone

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