BriClone is a conditioned medium collected from a human cell
line, supplied frozen in 100ml aliquots. It is a cloning medium suitable for
use in the post fusion stages of hybridoma production and for cloning hybridoma
cells under conditions of limiting dilution. When added to culture media,
BriClone replaces the function of feeder cells.
BriClone Hybridoma Cloning and Selection Supplement
(25ml)
Product Code: ZKBRICLONESAMPLE
Product Code: ZKBRICLONESAMPLE
BriClone Hybridoma Cloning and Selection Supplement (100ml)
Product Code: ZKBRICLONE
Product Code: ZKBRICLONE
Why use BriClone?
The use of feeder cells to support the outgrowth of
hybridomas has several disadvantages. These include the potential problem of
batch to batch variation in the quality of feeder cells, plus feeder cells
compete with freshly fused hybridomas resulting in the depletion of vital
nutrients from the culture medium. BriClone has been developed to overcome
these common problems that occur with feeder cells.
Benefits:
- Increased
efficiency: BriClone is more efficient then feeder cells in cloning by
limiting dilution methods
- Reduces
risk of culture contamination
- Eliminates
batch variation
- No
depletion of nutrients by feeder cells
- No
time consuming preparations
- Mycoplasma free
An example of hybridoma cloning using BriClone (5%) or mouse macrophages as
feeder cells. The hybridomas were cloned by limiting dilution at 1 cell/well.
Call the Caltag Medsystems office now on +44 (0)1280 827 460
or email office@caltagmedsystems.co.uk
to order your Briclone
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