Wednesday, 17 October 2012

Cerebrospinal Fluid Stabilisation with TransFix® EDTA

Cerebrospinal Fluid (CSF) can be stabilised and stored in TransFix® / EDTA for up to 10 days allowing samples to be batched and transported safely.
Juan-Manuel Sancho et al, used TransFix® EDTA to stabilize CSF. A further report (awaiting publication) by Dr. Maryalice Stetler-Stevenson (1) located at NIH in the USA concludes diagnosis of Leukaemia in CSF samples is dependent on the stability of the cells and that up to 30% of positive diagnoses were not being reported in non stabilised samples.
Most CSF samples are paucicellular and contain a limited number of cells with a rapidly decreasing viability. Evaluation of immunophenotyping in the study of CSF samples highlighted the need to stabilise samples to allow them to be safely stored and transported.
Sandra Quijano (2009) Journal of Clinical Oncology, 27, 1462 - 1469 - demonstrated that TransFix/EDTA effectively stabilises CSF samples for up to 10 days. Interpretation of cytological findings may be difficult because of the paucity of cells in CSF and the morphological similarities between benign and malignant cells. The study showed that flow cytometry is more sensitive and specific analytical method than cytomorphological analysis for the examination of CSF samples.
Sample preparation for flow cytometry:
  1. Place between 3ml and 5ml of CSF into a TransFix / EDTA CSF storage tube and mix by inversion 5-10 times.
  2. Store at 2 - 8ºC for up to 10 days.
  3. Allow samples to return to room temperature prior to flow cytometry and ensure that cells are resuspended by inverting the vial 30x.
Read more on TransFix® here.

Samples are available for all products, please request your samples by calling +44 (0) 1280 827460 or emailing 
cytomark@caltagmedsystems.co.uk.

No comments:

Post a Comment

Webmaster: AnnWebCom | Site design: custardcreative.co.ukCustard Creative Copyright 2005-2015 Caltag-Medsystems Limited - all rights reserved