Thursday, 12 July 2012

DJ-1/PARK7 - A Human ELISA Kit

DJ-1/PARK7  (Parkinson disease [autosomal recessive, early onset] 7 ) has been isolated as a gene associated with early-onset Parkinson's disease. DJ-1 is also involved in many diverse biological processes. Evidence suggests that DJ-1 plays a role in the oxidative stress response, modulates various transcription factors, DJ-1 also plays a role in human tumourigenesis. 
Recent Citation: Manuel F. Bande, et. Al., Invest. Ophthalmol. Vis. Sci., Jan 2012; 53: 62 - 67.

CircuLex TM Human DJ-1/PARK7 ELISA Kit, 96 wells.

Product Code: CY-9050Download datasheet here

DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming NIH-3T3 cells in cooperation with H-ras (1), a protein expressed in sperm (2), and a regulator of RNA-protein interactions (3). DJ-1 has also been isolated as a gene associated with autosomal early-onset Parkinson's disease (PD) (4). 


Taken together, DJ-1 appears to be involved in diverse biological processes (5). First, several lines of evidence suggest that DJ-1 plays a role in the oxidative stress response (6, 7). In cu1tured mammalian ce11s, DJ-1 quenches reactive oxygen species and is converted into a variant with a more acidic isoelectric point (6, 8). Therefore, DJ-1 protects against reactive oxygen species-induced cell death, and its suppression with small interfering RNA (siRNA) sensitizes cells to such insults (7-10). Second, DJ-1 modulates transcription through interaction with DJ-1-binding protein (11) as well as with protein inhibitor of activated STAT (PIAS) (12). The latter modulates the activity of various transcription factors, Third, DJ-1 has been recognized as a regulatory subunit of an RNA-binding protein (13). Fourth, DJ-1, which is structurally related to the molecular chaperone Hsp31, may have chaperone activity itself, preventing heat-induced aggregation of substrate proteins, including alpha-synuclein (14).


In addition, evidence also suggests that DJ-1 plays a role in human tumorigenesis. Breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to healthy and non-breast cancer patients (15). Furthermore, DJ-1 protein is increased in primary non-small cell lung carcinoma samples (16). Additionally, treatment of cells from the human lung cancer cell line NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression (16).


References: 

1. D. Nagakubo, T. Taira, H. Kitaura, M. lkeda, K. Tamai, S.M. Iguchi-Ariga and H. Ariga: Biochem. Biophys.Res.Commun.231, 509-513, 1997 
2. A. Wagenfeld, J. Gromoll and T.G, Cooper Biochem. Biophys. Res. Commun. 251, 545-549, 1998 
3. Y. Hod, S.N. Pentyala, T.C. Whyard and M.R. El-Maghrabi: J. Cell. Biochem. 72, 435-444, 1999 
4. Bonifati, V., Rizzu, P., van Baren, M. J., Schaap, O., Breedveld, G. J., Krieger, E., Dekker, M. C. J, Squitieri, F., Ibanez, P., Joosse, M., van Dongen, J.W., Vanacore, N., van Swieten, J.C, Brice, A., Meco, G., van Duijng, C. M., Oostra, B. A., Heutink, P.: Science 299, 256-259, 2003. 
5. Cookson, M. R, : Neuron 37, 7-10, 2003 
6. Mitsumoto, A.& Nakagawa, Y.: Free Radica1 Res.35, 885-893, 2001 
7. Canet-Aviles, R. M., Wilson, M. A., Mil1er, D. W., Ahmad, R., McLendon, C., Bandyopadhyay, S., Baptista, M. J., Ringe, D., Petsko, G. A. & Cookson, M. R.: Proc. Natl. Acad. Sci. USA 101, 9103-9108, 2004 
8. Taha, T., Saito, Y., Niki, T., Iguchi-Ariga, S.M., Takahashi, K. & Ariga, H.:, EMBO Rep.5, 213-218, 2004 
9. Martinat, C., Shendelrnan, S., Jonason, A., Leete, T., Bea1, M, F., Yang, L., Floss, T. & Abeliovich, A.: PLoS Biol, 2, e327, 2004 
10.Yokota, T., Sugawara, K., Ito, K., Takahashi, R., Ariga, H.& Mizusawa, H. :Biochem Biophys.Res. Commun, 312, 1342-1348, 2003 
11. Niki, T., Takahashi-Niki, K., Taira, T., Iguchi-Ariga, S. M. & Ariga, H.: Mol. Cancer Res. 1, 247-261, 2003 ,
 12. Takahashi, K., Taira, T., Niki, T., Seino, C., lguchi-Ariga, S. M. & Ariga, H.: J. Biol. Chern. 276, 37556-37563, 2001 
13. Hod, Y., Pentyala, S.N., Whyard, T.C.& El-Maghrabi, M.R.: J. Cell Biochem.72, 435-444, 1999 
14. Shendelman, S., Jcmason, A., Manimag, C., Leete, T. & Abeliovich, A.: PLoS Biol.2, e362, 2004 
15. F. Le Naour, D.E. Misek, M. C. Krause, L. Deneux, T. J. Giordano, S. Scholl and S.M. Hanash: Clin. Cancer Res. 7, 3328-3335, 2001 
16. J.P. MacKeigan, CM. Clements, J.D, Lich, R. M. Pope, Y. Hod and J.P. Ting: Cancer Res. 63 6928-6934, 2003

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1 comment:

  1. Thanks a lot for this contribution! It's been very useful for me. Everything is very open and represents very clear explanation of issues. Really blogging is spreading its wings quickly. Your write up is a good example of it. Your website is very useful. Thanks for sharing DJ-1/PARK7 - A Human ELISA Kits.

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