Thursday, 19 July 2012

Blood Controls for Flow Cytometry


The quantitation of CD4+ cells in human blood by flow cytometry provides a vital tool in evaluating many human immunodeficiencies.
CD4 blood controls developed by Cytomark are stabilised preparations of human whole blood with known CD4 absolute counts. They are intended as daily run controls to ensure that sample processing, equipment and operators are all performing optimally thereby providing quality assurance in the immune monitoring of patients. 

Benefits of CD4 Blood Controls

- Available in two formats, CD4 Low and CD4 Normal
- Verification of monoclonal antibody and FACS reagents
- Validation of flow cytometer performance
- Validation of operator technique
- Verification of red blood cell lysis
Technology and Preparation of CD4 Blood Controls
CD4 stabilised blood controls are prepared using the proprietary CytoFix® blood stabilisation technology. The stabilisation process is robust resulting in controls that are stable for up to 90 days when stored at 2 - 8°C.

Two CD4 stabilised blood controls are available; a normal CD4 blood cell profile (product number: CF04-N) and a low CD4 profile (product number: CF04-L). These controls can be purchased separately or as a pair (CF04-Duo). Each control is 1.5ml in volume, evaluation samples are available, please contact us for details.
In the controls, the white cell profile is adjusted to give a specific CD4+ count. The normal CD4 control (CF04-N) has a CD4+ absolute count within the normal healthy range (between 410 and 1590 cells/µl of blood). The low CD4+ control (CF04-L) has a CD4+ absolute count of between 200 and 300 cells/µl, which simulates CD4 levels in advanced HIV/AIDS patients.
CD4 blood controls prepared using the Cytofix® technology can also be used in External Quality Assurance (EQA) programmes due to their exceptional stability.
Read more on Blood Controls here.
Samples are available for all products, please request your samples by calling +44 (0) 1280 827460 or emailing cytomark@caltagmedsystems.co.uk.

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