Monday, 22 August 2011

QuantiGene® ViewRNA ISH Cell Assay for Fluorescent RNA In Situ Hybridization (RNA FISH)

QuantiGene ViewRNA Assays are novel in situ hybridization (ISH) assays enabling localization and quantitation with single-copy RNA sensitivity at single cell resolution in cells or tissues in singleplex and multiplex assay formats.

Fluorescent based in situ hybridization (FISH) techniques are used to visualize DNA or localized RNAs within cells. However the fluorescent in situ hybridization of RNA has always been limited by low sensitivity, complicated workflow and the inability to perform multiplex analysis. QuantiGene ViewRNA is a novel non-radioactive RNA fluorescent in situ hybridization solution that offers single-copy per cell mRNA sensitivity for one to four RNA targets. Affymetrix Research-Use-Only (RUO) products for RNA FISH analysis allow investigators doing biomarker discovery or clinical research to validate and study RNA biomarkers in suspension or adherent cells.

Unique Benefits

  • Sensitive in situ hybridization assay – detect one mRNA copy/cell
  • Multiplex in situ hybridization – study one to four mRNA targets per cell
  • Flexible probe set design – to target any mRNA sequence
  • Fast results – one day assay
  • Fluorescent signal – use of fluorescent microscope (no radioactivity involved)
  • Robust, reproducible results in adherent or suspension cells


  • Analysis of transcriptional heterogeneity
  • Cancer and other disease research
  • Biomarker validation
  • Neurobiology/neuroscience
  • Infectious diseases/virology
  • Stem cells and differentiation research
  • RNAi knockdown quantitation
  • Study of non-coding RNAs
  • An alternative to immunofluorescence (IF), immunocytochemistry (ICC) or DNA FISH assays

This assay has two required components - one assay kit and the target specific probe sets, each sold separately:

  • QuantiGene ViewRNA ISH Cell Assay Kit (Product Code: QVC0001)
  • QuantiGene ViewRNA Probe Sets - target specific probes (TYPE 1, 4, 6 and/or 10; download list of available probes PDF here

Download the product documentation here.

1 comment:

  1. I'm short of words to express the goodness of God in my life. I was diagnosed of herpes simplex virus 4 years ago. Which was the worst time in my life,cos of my career and i'm just 28th. I became worried,distress,felt so rejected,dirty and pity. I started keeping in touch with many hospitals,tried many solutions,and consulted many medical practitioners to get ride of my HSV virus,none of the ativiral treatment could cure me .I started trying fruits and herbs, and with time became very interested in herbs for solutions,to get ride of my HSV. I thought my life was gonna end there, i became worried and couldn't sleep at night. That faithful Sunday morning, friend of mine crystal who visited me home came up with a good news with her HIV/ AIDS test result negative. I was shocked,but so happy to have seen that. I wouldn't believe if I haven't seen the result and that would have being my worst mistake in life. She told me it was the work of an herbalist in Africa,Dr.Abang. who prepared the HIV/AIDS cured and sent it to her husband through the DHL. I became very interest and went online to search for natural ways for my cure then I saw a testimony of a lady comments on how she was cured from cancer by the same herbalist, then,I quickly email him@ med.are very active with immune booster with no site him for the solution to all your sickness. He cures all viruses and diseases
    Is a God sent in my life....


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