Tuesday, 29 May 2012

TransFix® and Blood Controls from Cytomark

Two new flyers are available from Cytomark – TransFix® and Blood Controls for Flow Cytometry.

TransFix® is a preservative solution that maintains cellular integrity while at the same time preserving antigenicity. It can be used to preserve and stabilise body fluids including blood, CSF, bone marrow and fine needle aspirates. TransFix® treated samples are stable for a minimum of 10 days at 2-22°C and and for 4 days when stored at 37°C. For more information please view our previous blog on TransFix®.

Download the TransFix® flyer here.
Or email us to request your hard copy.

Blood Controls for Flow Cytometry 
CD4 blood controls developed by Cytomark are stabilised preparations of human whole blood with validated CD4 absolute counts. They are intended as daily run controls to ensure that sample processing, equipment and operators are all performing optimally thereby providing quality assurance of the immune monitoring of patients. For more information please view our previous blog on Blood Controls.

Download the Blood Controls flyer here.
Or email us to request your hard copy.

Read more on Cytomark here or visit the website: www.cytomark.co.uk

mSIN3A and the regulation of cell proliferation and differentiation

The bHLHZIPP protein Max interacts with both the Myc and Mad family proteins to form heterodimers that specifically bind DNA and regulate transcription. Myc-Max complexes act as transcriptional activators, while Mad-Max complexes have the opposite function.

mSin3A is a mammalian homolog of the Saccharomyces cerevisiae transcriptional corepressor Sin3p. mSIN3A binds to the Mad/Max family of transcription factors via the first N-terminal 25 residues of Mad, known as the mSin3 interaction domain, or SID. The Mad/Max heterodimer and mSin3A form ternary complexes that bind DNA and modify Mad activity, and are thus involved in regulating cell proliferation and differentiation.

Anti-mSIN3A Polyclonal Antibody
Product Code: BMP004
Download the datasheet here.

Recent Citation: Pengpeng Ma, et. Al., PNAS, Feb 2012; 109: E481 - E489

Caltag Medsystems is the exclusive UK distributor for MBL products. For further information on the MBL product range, please visit www.mblintl.com or request a copy of the MBL reference guide.

ELISA Kit for Gelsolin (GS)

Gelsolin is an actin-regulatory protein that modulates actin assembly and disassembly, and is believed to regulate cell motility in vivo through modulation of the actin network. In addition to its actin-regulatory function, gelsolin has also been proposed to affect cell growth.It is first isolated from rabbit lung macrophages as a modulator of the cytoplasmic actin gel−sol transformation. Gelsolin can sever actin filaments and cap the fast-growing ends of the filaments in vitro; this promotes actin disassembly. Gelsolin also has the ability to nucleate actin polymerization. These functions are activated by Ca2 and inhibited by polyphosphoinositides (PPIs). Such observations have led to the view that gelsolin regulates actin reorganization in response to changes in the concentrations of Ca2 and PPIs in living cells.

Product Code: E90372Hu
Download product datasheet here.

ELISA Kit for Gelsolin is also available in Mouse, Rat and Monkey. Please contact us if you are interested in these products.

We offer the lowest prices on Uscn ELISAs – and Bulk Discounts! Click here for more information. 

Caltag Medsystems is the UK distributor for Uscn and they currently have over 4,600 ELISA Kits in their portfolio. Search the complete Uscn product range by product type and/or species reactivity in excel format here.

PNA Mutation Detection Kits

PNA is a synthetic oligonucleotide with peptide backbones instead of sugar groups. It binds to its complementary DNA template sequence with a higher affinity and specificity than DNA-DNA binding. The resulting PNA-DNA duplex is not recognized by DNA polymerases and thus makes it the ideal clamp to inhibit PCR of the wtDNA and allows for amplification of the unobstructed mutated DNA sequences. The PNA clamps are sensitive enough to discriminate against single-base mismatches in nucleotides and are considered as one of the best options for researches on DNA diagnostics.

Abnova’s PNA Mutation Detection Kit uses optimized PNA clamps that bind tightly to their complementary DNA sequences and have high stability for inhibition of PCR amplification. This allows for amplifications of only the mutant target DNA in the mixture of major wild-type and minor mutated DNA sequences. There are 3 PNA Mutation Detection Kits available, targeting KRAS, BRAF, and EGFR oncogenes and their somatic mutations individually. KRAS and BRAF genes are involved in several cancers, including colorectal, thyroid, and lung cancers. EGFR is highly related to non-small cell lung cancers which consist 80 – 85% of all lung cancers.

Try Abnova’s PNA Mutation Detection Kits and improve your researches with more rapid, sensitive, and accurate techniques/assays.

* Fast and accurate detection of any mutation by real-time PCR
* High reproducibility and compatibility with real-time PCR machines
* High sensitivity (<1% of mutated DNA sequence can be detected)
* Convenient formulated master reagent mixtures
* Easy test protocol and total procedure takes <3 hours
* Only small amount of DNA sample needed (approx. 1 ng)

Download more information please view our website.

Monday, 21 May 2012

Cytomark - Flow Cytometry Reagents

Cytomark is a UK Biotechnology Company formed to bring to market blood stabilisation technologies developed by UK NEQAS who are responsible for External Quality Assurance in Blood Pathology in the UK and many other countries in Europe.
Cytomark have two blood stabilisation technologies:

  • TransFix® – A Cellular stabilisation Reagent.

TransFix® is a CE marked cellular stabilisation solution that when added to Whole Blood, Cerebrospinal Fluid (CSF), Bone Marrow and Fine Needle Aspirates, preserves cell surface antigens for a minimum of 10 days. 
TransFix® is added to whole blood samples at the time of venepuncture and provides stabilisation to permit extended storage and/or transportation. This allows greater flexibility for testing and potential reduction in costs e.g. by allowing samples to be batched.
TransFix® is available in several formats: blood or CSF sample storage tubes, direct draw vacuum blood collection tubes and in bulk volumes. 
Read more on TransFix® here. 

  • Blood Controls for Flow Cytometry
The quantitation of CD4+ cells in human blood by flow cytometry provides a vital tool in evaluating human immunodeficiencies. Cytomark have developed two stabilised preparations of human blood with known CD4 absolute counts. They are intended for use as daily run controls in blood sample analysis.
CD4 Blood Controls are available in two formats; CD4 Normal - between 410 and 1590 cells/µl and CD4 Low - between 200 and 300 cells/µl, which simulates CD4 levels in advanced HIV/AIDS patients.
Read more on Blood Controls here.

Samples are available for all products, please request your samples by calling +44 (0) 1280 827460 or emailing cytomark@caltagmedsystems.co.uk.

Visit the Cytomark website for further information. 

Friday, 11 May 2012

Atg16L - a Crohn's disease susceptibility gene

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg16L-Atg12-Atg5 forms a complex that elongate autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane. It has been shown that ATG16L1 is a Crohn's disease susceptibility gene.
Immunocytochemical detection of Atg16L on 4% PFA fixed nutrient normal rat kidney cell  line (NRK, left) and starved NRK (right) with PM040.

Anti-Atg16L Polyclonal Antibody, 100ug - Product Code: PM040
Anti-Atg16L Polyclonal Antibody, 10ul - sample size - Product Code: PM040B
Download the data sheet here
Suitable for use in western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry and flow cytometry

Recent Citation: Kevin Moreau, et. Al., J. Cell Biol., Feb 2012; 196: 483 – 496

Caltag Medsystems is the exclusive UK distributor for MBL products. For further information on the MBL product range, please visit http://www.mblintl.com or request a copy of the MBL reference guide.

ELISA Kit for Lumican (LUM)

Lumican, also known as LUM, is a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings.

- Product Code: E91496Hu

Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.
Download the product datasheet here.

We offer the lowest prices on Uscn ELISAs – and Bulk Discounts! Click here for more information. 

Caltag Medsystems is the UK distributor for Uscn and they currently have over 4,600 ELISA Kits in their portfolio. Search the complete Uscn product range by product type and/or species reactivity in excel format here.

ACE2 - Implicated in Hypertension and Diabetes

The renin-angiotensin system (RAS) regulates system blood pressure and renal function. Angiotensin-converting enzyme 2 (ACE2) is an enzymatically active homolog of ACE, being highly specific for angiotensin cleavage. ACE2 has direct effects on cardiac functions. It is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE2 converts angiotensin I to angiotensin 1-9. ACE2 was also identified as a functional receptor for SARS coronavirus.

It has been shown that ACE2 is implicated in pathophysiological states, including myocardial ischaemia, heart failure, renal failure, atherosclerosis, and diabetes. If ACE2 is contributing to the development of hypertension is currently under investigation.

ACE2 (human) (rec.) - AG-40A-0048-C010
ACE2 (human), mAb (AC18F) – AG-20A-0032-C050
ACE2 (human), pAb – AG-25A-0042-C100
ACE2 (human) ELISA Kit – AG-45A-0022EK-KI01
ACE2 (human) (IntraCellular) ELISA Kit – AG-45A-0039EK-KI01

Adipogen products are available in the UK exclusively through Caltag Medsystems. See the full range of Adipogen’s ACE2 products, including antibodies, ELISA’s and proteins here

Abnova - Kit Collection

When it comes to bioassay methods, most people think of immunoassays right away. Immunoassays are tests used to quantify or identify a particular substance in a sample by measuring the specific binding of antibody-antigen complexes and to detect them via an indicator reaction. Most widely used immunoassay methods are ELISAs. Abnova has developed 1,000+ ELISA kits targeting a variety of research areas, such as apoptosis, autoimmune diseases, signal transduction, and many more. 
Don't forget there are more bioassay formats available than the common ELISAs. Abnova now carries 500+ assay kits in a variety of formats including screening kits and detection assays to measure the activities of analytes. These kits have enabled high assay sensitivity with an emphasis on superior analytical characteristics and user-friendly kit performance. Each kit has gone through rigorous validation and stability tests to ensure high precision, accuracy, sensitivity, and specificity. With the various assay formats available, we have made it easier for you to select from 8 categories:
Activity Assay | Apoptosis | Cell Damage and Oxidative Stress | Cell Proliferation and Cytotoxicity | Metaboliam | Epigenetic | Fluorescent Protein | Signal Transduction | 

Abnova are continually expanding their kit portfolio to meet customers' needs. Abnova provides a total solution for antibody applications for quantitative measurement of your protein of interest, so you can focus on more important parts of your research instead of the arduous task of searching and testing for the matching antibody components.

Please contact us if there is a kit you are looking for and we will find it for you!

Autoimmunity - Skin Blistering Disease

Pemphigus is an autoimmune blistering disease that targets skin and mucous membrane, caused by autoantibodies to cell surface antigens of stratified epithelia of mucous membranes and skin. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1 (Dsg1) in PF and desmoglein 3 (Dsg3) in PV. Pemphigus patients with both mucosal and skin lesions have antibodies to both Dsg1 and Dsg3.

Bullous Pemphigoid (BP) is an autoimmune disease of the skin and mucous membranes. Skin lesions of BP patients show separation of basal keratinocytes from the underlying dermis leading to subepidermal blisters. Patients with BP have circulating IgG autoantibodies against components of hemidesmosome, and target two distinct molecules, BP230 and BP180. BP180 is considered the target molecule to which pathogenic IgG autoantibodies bind.

Epidermolysis Bullosa Acquisita (EBA) is a chronic subepidermal blistering disease of the skin and mucous membranes. EBA is characterised by the presence of (IgG) antibodies targeting the NC1 domain of Type VII collagen. MBLI has developed a highly specific, highly sensitive ELISA kit for the detection of the NC1 and NC2 domain of Type VII Collagen. 

Related products from MBL International:
MESACUP Desmoglein Test Dsg1 (Product Code: RG-7680EC-D)
MESACUP Desmoglein Test Dsg3 (Product Code: RG-7685EC-D)
BP180 ELISA Kit (Product Code: RG-7695-EC-D)
BP230 ELISA Kit (Product Code: RG-7613EC-D)
Anti-Type VII Collagen ELISA Kit (CE Mark) (Product Code: RG-7845E)

Take a look at MBL’s handy new reference guide here.

Tuesday, 1 May 2012

ELISA Kit for Elastin (ELN)

Elastin is a protein in connective tissue that is elastic and allows many tissues in the body to resume their shape after stretching or contracting. Elastin helps skin to return to its original position when it is poked or pinched. Elastin is also an important load-bearing tissue in the bodies of mammals and used in places where mechanical energy is required to be stored.Elastin is primarily composed of the amino acids glycine, valine, alanine, and proline.
It is a specialized protein with a molecular weight of 64 to 66 kDa, and an irregular or random coil conformation made up of 830 amino acids.Elastin is made by linking many soluble tropoelastin protein molecules, in a reaction catalyzed by lysyl oxidase, to make a massive insoluble, durable cross-linked array. The amino acid responsible for these cross-links is lysine.
Download the product datasheet here. 

ELISA Kit for Elastin is also available in Mouse and Rat. Please contact us if you are interested in these products.

We offer the lowest prices on Uscn ELISAs – and Bulk Discounts! Click here for more information

Caltag Medsystems is the UK distributor for Uscn and they currently have over 4,600 ELISA Kits in their portfolio. Search the complete Uscn product range by product type and/or species reactivity in excel format here.

CLUSTERIN - Potential Biomarker of Cancers and Type 2 Diabetes

Clusterin (Apolipoprotein J; ApoJ; SP-40,40; Sulfated glycoprotein-2; SGP-2) is a heterodimeric highly conserved disulfide-linked multifunctional glycoprotein being expressed in a wide variety of tissues and secreted in all human fluids. Clusterin is supposed to have a protective role against human atherosclerosis. In patients with type 2 diabetes mellitus serum clusterin levels are significantly higher than in healthy individuals. Furthermore, body fluids level of clusterin from patients with malignant tumors, such as breast cancer, bladder cancer and prostate cancer, are elevated. Variants of the CLU gene are associated with Alzheimer’s disease. CLU expression is increased in Alzheimer’s disease patients. In summary, clusterin likely provides a promising novel diagnostic marker in many major human diseases like diabetes, cancers or neurodegenerative diseases.

Clusterin (human) Competitive ELISA Kit

See the full range of Adipogen’s Clusterin products, including antibodies and proteins here

Caltag Medsystems is the exclusive UK distributor for Adipogen products. For further information on the Adipogen product range click here.

Tools For DNA Screening Studies

Fluorescence In Situ Hybridization (FISH) Probes
FISH (Fluorescence In Situ Hybridization) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. This technique is based on the mechanism of nucleic acid base pairing. Characterized by high degree of sequence complementarity, only the exact matching parts of the chromosome will be recognized and bound by fluorescent probes. This powerful technique enables researchers to identify a range of chromosomal aberrations across the genome in a short time, including those causing mental retardation, various cancers, birth defects, etc.

Abnova now carries 6 different lines of FISH Probes and other accessory reagents that sum up to a comprehensive collection of over 600 products. Gene amplification, gene loss, break-apart of a gene, genes fusion, or chromosomal aneuploidy can be easily detected with convenience. These products are fast and sensitive, and works on metaphase spread, paraffin embedded and frozen tissue. Each FISH probe product has either a single, a pair, or multiple numbers of locus-specific, fluorophore-labeled probe(s) originated from a bacterial artificial chromosome (BAC) library.

Single Color FISH Probes
These products are provided in 5X concentrated format to allow mixing of up to 5 single color FISH probes in a single hybridization assay. Each probe has five different colors of choice, including FITC, Texas Red, Cy5, DEAC and R6G.
Dual Color FISH Probes
These products are ready to use, each has a pair of locus-specific, fluorophore-labeled probes with different colors. These products are used to detect gene amplification, gene loss, gene split and gene translocation.
Multiple Color FISH Probes
The products in this category each comprise of different combinations of fluorophore-labeled probes specific for chromosomes 13, 18, 21, X, and/or Y.
Start your FISH studies today! Use the best DNA Screening tools available from Abnova!

Anti-PIP3 [phosphatidyl inositol-3,4,5-triphosphate] Monoclonal Antibody

PIP3 plays multiple roles in regulating cell growth and survival.  Approximately 50% of all cancers possess mutations in genes that regulate PIP3 production, resulting in cells with abnormally high levels of PIP3. PIP3 acts as a critical second messenger in insulin signalling; and is also involved in T cell activation via CD28, regulating exocytosis, and inducing actin reorganization and membrane ruffling.

Recent Citation: Melkam Kebede, et. Al., PNAS, Feb 2012; 109: 2376 - 2381 
Anti-PIP3 [phosphatidyl inositol-3,4,5-triphosphate] Monoclonal Antibody, 100ul

Product Code: D145-3

Download datasheet here

Caltag Medsystems is the exclusive UK distributor for MBL products. For further information on the MBL product range, please visit http://www.mblintl.com or request a copy of the MBL reference guide.

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