Tuesday, 24 April 2012

46 Multiplex Tyrosine Capture ELISA Assay

Receptor tyrosine kinases (RTKs) play important roles in converting signals from extracellular to intracellular.  Tyrosine autophosphorylation generates recruitment sites for downstream signalling proteins containing Src homology-2 (SH2). Binding of different SH2 domain-containing signalling proteins represents the activation of different signalling pathways. The SH2 domain-based RTK profiling kit from Signosis is an ELISA-like assay, allowing the simultaneous measurement of 46 SH2 domain bindings using either cell lysate or peptides.  This allows the activation of signalling pathways to be assessed through quantitative examination of SH2 domain binding. 


Benefits:

  1. Multiplex - A single assay measures the interaction of RTK with 46 SH2 domains.
  2. Quantitative - The difference between individual proteins in the interaction of the SH2 domains can be quantitatively compared. 
  3. Simple - The assay is performed using the same procedure as an ELISA.

SH2 Domain-Based RTK Profiling Kit. Product Code: DA-0001
Read More on the Signosis Web Site 
User Manual

Friday, 20 April 2012

ELISA Kit for Hepcidin (Hepc)


Hepcidin is a peptide hormone produced by the liver discovered in 2000, that appears to be the master regulator of iron homeostasis in humans and other mammals. In humans, HAMP is the gene that encodes for hepcidin. 

- Product code: E91979Mu
- Product code: E91979Hu
- Product code: E91979Ra
- Product code: E91979Po
- Product code: E91979Bo

Hepcidin preprohormone, prohormone and hormone size are 84, 60 and 25 amino acids respectively. 20 and 22 amino acid forms of Hepcidin also exist in the urine. The N terminal region is required for function, and deletion of 5 N-terminal peptides results in a loss of function. Hepcidin has a beta-sheet rich structure with 4 disulfide bonds. Hepcidin directly inhibits ferroportin, a protein that transports iron out of cells that store it. This prevents enterocytes of the intestines from secreting iron into the hepatic portal system, reducing iron absorption.

We offer the lowest prices on Uscn ELISAs – and Bulk Discounts! Click here for more information
Caltag Medsystems is the UK distributor for Uscn and they currently have over 4,600 ELISA Kits in their portfolio. Search the complete Uscn product range by product type and/or species reactivity in excel format here.

PCSK9 : an attractive drug target for lowering LDL-C?

PCSK9 plays a major role in cholesterol metabolism through posttranslational down regulation of LDLR, the receptor responsible for clearing LDL-C from plasma.
PCSK9 (neural apoptosis-regulated convertase, NARC-1) is a 692-residue extracellular protein representing the 9th member of the secretory subtilase family expressed primarily in the kidneys, liver and intestines.

Genetic studies mapped PCSK9 along with LDLR and APOB to cause autosomal dominant hypercholesterolemia (ADH). Gain-of-function mutations increased plasma levels of low-density lipoprotein cholesterol (LDL-C), whereas nonsense or missense (loss-of-function) mutations, which interfere with folding or secretion of PCSK9, led to a reduction of plasma levels of LDL-C and an 88% decrease in the risk of coronary heart disease (CHD).
These findings led to a hypothesis that PCSK9 exerts its role in cholesterol metabolism through posttranslational down-regulation of LDLR, the receptor responsible for clearing plasma LDL-C. The hypothesis is consistent with a secreted form of PCSK9 bound directly to the first epidermal growth factor-like repeat (EGF-A) of LDLR and resulting in degradation of the receptor. PCSK9 appears to be an attractive drug target for lowering LDL-C.

PCSK9 Products:
CircuLex Human PCSK9 ELISA Kit - Product Code CY-8079
CircuLex Mouse/Rat PCSK9 ELISA Kit - Product Code CY-8078
CircuLex PCSK9-LDLR in vitro Binding Assay Kit - Product Code CY-8150

References:

1. Seidah NG, Benjannet S, Wickham L, Marcinkiewicz J, Jasmin SB, Stifani S, Basak A, Prat A, Chretien M (2003) Proc Natl Acad Sci USA 100:928
2. Abifadel M, Varret M, Rabes JP, Allard D, Ouguerram K, Devillers M, Cruaud C, Benjannet S, Wickham L, Erlich D, et al. (2003) Nat Genet 34:154
3. Leren TP (2004) Clin Genet 65:419
4. Allard D, Amsellem S, Abifadel M, Trillard M, Devillers M, Luc G, Krempf M, Reznik Y, Girardet JP, Fredenrich A, et al. (2005) Hum Mutat 26:497.
5. Cohen JC, Boerwinkle E, Mosley TH, Jr, Hobbs HH (2006) N Engl J Med 354, 1264
 6. Berge KE, Ose L, Leren TP (2006) Arterioscler Thromb Vasc Biol 26:1094
7. Maxwell KN, Breslow JL (2004) Proc Natl Acad Sci USA 101:7100
 8. Rashid S, Curtis DE, Garuti R, Anderson NN, Bashmakov Y, Ho YK, Hammer RE, Moon YA, Horton JD (2005) Proc Natl Acad Sci USA 102:5374
9. Lagace, T. A., Curtis, D. E., Garuti, R., McNutt, M. C., Park, S. W., Prather, H. B., Anderson, N. N., Ho, Y. K., Hammer, R. E., and Horton, J. D. (2006) J. Clin. Investig. 116, 2995
10. Cameron, J., Holla, O. L., Ranheim, T., Kulseth, M. A., Berge, K. E., and Leren, T. P. (2006) Hum. Mol. Genet. 15, 1551รข€“1558 11. Zhang, D. W., Lagace, T. A., Garuti, R., Zhao, Z., McDonald, M., Horton, J. D., Cohen, J. C., and Hobbs, H. H. (2007) J. Biol. Chem. 282, 18602

AdipoGen™ - The Original Source for New & Innovative Small Molecules

BioViotica, part of the Adipogen portfolio is dedicated to producing and characterizing “Rare Active Natural Products” as research reagents.


BioViotica focuses on the discovery and fermentation of rare biological active natural products from bacteria and fungus. These products have a defined mode of action and are of high interest to the academic, pharmaceutical high throughput and medicinal chemistry research covering biochemistry, molecular biology and pest management. The compounds are sold as single items or as Natural Product Libraries for screening purposes.

Small molecules recently introduced to the bench include:

New Sonic Hedgehog Modulator: Robotnikinin - Product Code: AG-CR1-0069-M001
Brain Permeable SIRT2 Inhibitor: AK-7 - Product Code: AG-CR1-3511-M005
Potent FXR Agonist: 5-ECDCA - Product Code: AG-CR1-3560-M005
New Promising Antibiotic Structure:Tropodithietic acid (TDA) - Product Code: BVT-0152-M001
Stem Cell Biology Modulator: Y-27632.2HCl - Product Code: AG-CR1-3564-M001
Hedgehog (Hh) Signalling Pathway Inhibitor: GANT61 - Product Code: AG-CR1-3561-M001
TRPV1 and TRPA1 Agonist: Hydroxy-alpha-sanshool - Product Code: AG-CN2-0005-M001
Selective Inhibitor of Pim-1 and Pim-2Protein Kinases: SMI-4a - ProductCode: AG-CR1-3503-M005
Programmed Cell Death Inducer: CHS-828 - Product Code: AG-CR1-0064-M005

Adipogen products are available in the UK exclusively through Caltag Medsystems. Please visit www.caltagmedsystems.co.uk or www.adipogen.com for further product information.

Isotype Control Antibodies

Isotype controls are most commonly used in flow cytometry experiments for delineating the non-specific background signals caused by the primary antibodies. Isotype control antibodies are designed to retain all the non-specific characteristics of the primary antibodies, except the specificity for target cells in the experiment. The purpose is to confirm the binding of the primary antibody with the target cell is specific and not a result of other non-specific bindings and protein interactions. Thus, isotype controls need to be matched exactly with the primary antibodies to the host species and the isotypes, including heavy chain and light chain. There are many immunoglobulin subclasses available for isotype controls. The isotype control immunoglobulin(Ig) subclasses are IgG1,IgG2a, IgG2b, IgG3 and IgM. Most isotype controls are monoclonal. However, when it comes to more than one IgG subclass, they are not suitable. Therefore, Abnova also offers several isotype control polyclonal antibodies for use inthese circumstances.

Abnova carries conjugated isotype control antibodies, and unlabeled monoclonal antibodies. The conjugation formats and staining conditions are also required to be identical in the experiment in order to accurately assess the level of specific binding. Choosing a conjugated antibody can save you a lot of time and efforts. However, as an alternative, choosing an unlabeled antibody will give you more flexibility for your experiment if you are using more than one dye.

Find out more about Abnova here.

Multiplex 31 Cytokines in 1 ELISA Assay

Cytokines are signalling molecules that have critical roles in many biological processes. Groups of cytokines are commonly involved in disease processes, and comprehensive analysis of the expression of multiple cytokines is paramount to revealing the underneath mechanism of the disease state effectively.

TheHuman Cytokine ELISA Plate Array I from Signosis, allows monitoring of the abundance of 31 human cytokines in a high-throughput manner. This assay is a fast and sensitive tool for quantitatively profiling the levels of multiple cytokines between samples simultaneously.

Benefits
  • Multiplex - AELISA plate allows the measurement of 31 cytokines for 3 samples.
  • High Sensitivity - The assay requires minimal amount of samples with chemiluminescence.
  • Quantitative Profiling - Unlike membrane-based array assays, the differences of individual proteins among samples can be quantitatively compared.
  • No need of capital equipment - Unlike beads-based assays, no capital equipment is required.
  • Simple procedure - All in one system with a pre-coated plate.


Human Cytokine ELISA Plate Array I (Chemiluminescence): Product Code EA-1011

We now supply Uscn products and our prices are the best in the UK!

Caltag Medsystems are pleased to bring you the lowest prices for the Uscn product range for ELISAs.


Prices on single kits are the best in the UK, but we’ve also included extra discounts for purchases of 2 or more products to provide even more value for money.  Just quote “Uscn Bulk” on your order to qualify.

Number of Kits
Discount Applied
1
List Price
2-4
5%
5-7
7.5%
8-10
10%
10+
Enquire


Uscn provide over 4,600 ELISA kits for targets across a wide variety of species including human, mouse, rat, fish, plant, horse and more!

Please contact us if you have any questions about Uscn products or the discounts.
Search the complete Uscn product range by product type and/or species reactivity in excel format here.

Wednesday, 11 April 2012

MBL Posters and Reference Guide Available



Take a look at MBL’s handy reference guide – go to the MBL supplier page to download it now. This guide contains information about some of MBL’s top products. The guide covers a selection of topics including Apoptosis, Cancer, Immunology, Epigenetics, Fluorescent Proteins, Metabolism and more. To request a hard copy of the reference guide please email us.





MBL also provide a selection of posters including:

If you would like to receive a hard copy of the reference guide or any of the posters, please email office@caltagmedsystems.co.uk requesting them.

Tuesday, 10 April 2012

Exbio - Design, Development and Manufacture of Monoclonal Antibodies


Exbio is a new addition to the Caltag Medsystems Portfolio.

Exbio design, develop and manufacture monoclonal antibodies and are a trusted and reliable supplier to leading antibody vendors.

The Exbio Product Portfolio can be divided into four main sections:

Flow cytometry validated antibodies for a broad range of research applications (including human and rodent immunology and cancer research), these antibodies are available in many colours and formulations. Click here to download a table of all flow cytometry antibodies in excel format.

Premixed antibody conjugate cocktails for in vitro diagnostic (CE IVD) applications (immunophenotyping of white blood cells). Click here for all Exbio IVD products.

Flow Cytometry assays (FlowEx® Kits) for comprehensive analysis of patients status (BasoFlowEx® for allergy, FagoFlowEx® for phagocytosis, SpermFlow Kit for human semen quality) and others for apoptosis, and endocytosis. Click here for further FlowEx® Kit information.

Monoclonal Antibodies for other applications (functional in vivo studies, immunohistochemistry, Western blotting, immunoprecipitation). Click here to view Exbio antiobodies and search by application.

Visit the Exbio website for full product details at www.exbio.cz.

Wednesday, 4 April 2012

RIP Assay Kits for the Immunoprecipitation of Ribonucleoproteins from Cell Extracts

MBL have developed two RIP assay kits which enable the immunoprecipitation of mRNA-RBP (RNA binding protein) complexes with RBP specific antibodies.
RIP-Chip works on the same principle as the widely used ChIP-chip. It is the immunoprecipitatation of ribonucleoproteins (RNP) from cell extracts using an antibody raised against the RBP of interest. This procedure is then followed by microarray analysis.
MBL provide 2 RIP assay kits:
The RIP-assay kit for RNA for the immunoprecipitation of mRNP complexes and isolation of mRNA
The RIP-assay kit for microRNA, for the immunoprecipitation of mRNP complexes and isolation of functionally related mRNAs and miRNAs. The kit provides 3 different protocols for either the 1) isolation of small RNAs 2) simultaneous isolation of both small and large RNAs 3) separate isolation of small and large RNAs .
For RIP-assay starter kits, see here , these kits contain a trial size of the RIP certified antibody (40 ug) and the full sized version the RN1001.
Principles of RIP-Assay Kit

1). Biological approach to find functionally related genes f disease pathways
2). Analyse post-transcriptional regulated genes
3). Establish novel components of cellular pathways
4). Identify new drug mechanisms
5). Identification of RNAs in RNP complexes can be done in a few hours
6). Largest selection of antibodies validated for RNA-binding protein immunoprecipitation


The RIP-assay kit for RNA Product Code: RN1001
The RIP-assay kit for microRNA Product Code: RN1005
Request the RiboCluster Profiler: Novel Tools for Post-Transcriptional Regulation of Gene Expression Research

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