FISH (Fluorescence In Situ Hybridization) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. This technique is based on the mechanism of nucleic acid base pairing. Characterized by high degree of sequence complementarity, only the exact matching parts of the chromosome will be recognized and bound by fluorescent probes. This powerful technique enables researchers to identify a range of chromosomal aberrations across the genome in a short time, including those causing mental retardation, various cancers, birth defects, etc.
Abnova now carries 6 different lines of FISH Probes and other accessory reagents that sum up to a comprehensive collection of over 600 products. Gene amplification, gene loss, break-apart of a gene, genes fusion, or chromosomal aneuploidy can be easily detected with convenience. These products are fast and sensitive, and works on metaphase spread, paraffin embedded and frozen tissue. Each FISH probe product has either a single, a pair, or multiple numbers of locus-specific, fluorophore-labeled probe(s) originated from a bacterial artificial chromosome (BAC) library.
Single Color FISH Probes
These products are provided in 5X concentrated format to allow mixing of up to 5 single color FISH probes in a single hybridization assay. Each probe has five different colors of choice, including FITC, Texas Red, Cy5, DEAC and R6G.
- Chromosome FISH Probes: Detect abnormal numbers of chromosomes (Aneuploidy)
- Subtelomere FISH Probes: Screen for subtelomeric chromosome abnormality
These products are ready to use, each has a pair of locus-specific, fluorophore-labeled probes with different colors. These products are used to detect gene amplification, gene loss, gene split and gene translocation.
- Gene FISH Probes: Identification of specific gene amplification / gene loss
- Split FISH Probes: Identification of gene split (break-apart of a gene)
- Translocation FISH Probes: Identification of gene translocation (gene fusion)
The products in this category each comprise of different combinations of fluorophore-labeled probes specific for chromosomes 13, 18, 21, X, and/or Y.
- Prenatal FISH Probes: Investigation of genetic chromosomal syndromes