Friday, 9 March 2012

AMP Kinase Assay Kit for measurement of endogenous AMPK activity

The AMP Kinase Assay Kit from MBL is designed to measure the activities of purified AMP-activated protein kinase (AMPK) or recombinant AMPK for the rapid and sensitive evaluation of activators or inhibitors.

The phospho-serine specific monoclonal antibody used in this assay kit has been demonstrated to recognize the phospho-serine 789 in mouse Insulin Receptor Substrate-1 (IRS-1), which is efficiently phosphorylated by AMPK. The AMPK Assay kit can be used for column fractions of cultured primary cell, cell line, or tissue can be assayed.

CycLex AMPK Kinase Assay Kit. Product Code: CY-1182

  1. Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK. Lin YY et al. Nature. 2012 Feb 8; 482(7384): 251-5.
  2. Identification of aneuploidy-selective antiproliferation compounds. Tang YC et al. Cell. 2011 Feb 18;144 (4):499-512.
  3. Peptide switch is essential for Sirt1 deacetylase activity. Kang H et al. Mol Cell. 2011 Oct 21;44(2):203-13.
Further Information:
AMP-activated protein kinase (AMPK) regulates cellular energy metabolism by phosphorylation of key enzymes in metabolic pathways, such as acetyl–coenzyme A carboxylase (ACC) or mTOR (mammalian target of rapamycin), by modulating their activities, and by regulating the activity of transcription factors and transcriptional cofactors. Its functions have been extensively studied in muscles and liver. AMPK stimulates pathways, which increase energy production (glucose transport, fatty acid oxidation) and switches off pathways, which consume energy (lipogenesis, protein synthesis, gluconeogenesis). Activation of AMPK in adipose tissue can be achieved through situations such as fasting and exercise. Adiponectin and leptin as well as hypoglycaemic drugs are activators of adipose tissue AMPK. This activation probably involves changes in the AMP/ATP ratio, and the upstream kinase LKB1. These results have led to the concept that AMPK has an interesting pharmaceutical potential in situations of insulin resistance (type 2 diabetes) and it is indeed the target of existing drugs, e.g. Metformin and hormones which improve insulin sensitivity.

Mammalian AMPK is a trimeric enzyme comprising a catalytic α subunit (63 kDa) and non-catalytic β and γ subunits. Multiple isoforms of each mammalian enzyme exist (α1, α2, β1, β2, γ1, γ2, γ3), each encoded by a different gene. The β subunits have a calculated molecular mass of 30 kDa, but migrate on SDS-PAGE with apparent masses of 38 kDa (β1) and 34 kDa (β2), whereas the three γ isoforms have molecular masses of 37 kDa (γ1), 63 kDa (γ2) and 55 kDa (γ3). While AMPK complexes containing α2 (the first catalytic isoform to be cloned) predominate in skeletal and cardiac muscle, approximately equal levels of α1 and α2 complexes are present in the liver. In contrast, pancreatic islet β-cells largely express α1 complex activity.

CycLex AMPK Kinase Assay Kit. Product Code: CY-1182


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