Monday, 19 December 2011

New Application for Analyzing Functionally Related miRNAs

New technology to isolate and analyze intact RBP-mRNA-miRNA complexes.
RIP-Chip (ribonucleoprotein immunoprecipitation-microarray profiling) is a biochemical approach to identify the composition and organization of endogenous mRNAs, miRNAs and RNA binding proteins (RBPs) within messenger ribonucleoprotein (mRNP) complexes. RIP-Chip has been successfully employed to isolate AGO-containing RNPs by immunopurification with anti-AGO antibodies. When the co-isolated miRNA and mRNA subpopulations are analyzed using the computational predictions of conserved seed sequences, this approach provides a powerful tool to identify functional miRNA targets based on their physical interaction in vivo. RBPs have been reported to bind to mRNAs that encode functionally related proteins, and coordinately regulate these mRNAs during cellular processes. The RIP-Chip approach can isolate functionally related mRNAs. Since miRNAs can be co-immunoprecipitated with those mRNAs, the RIP-Chip approach can also isolate miRNAs that regulate specific group of mRNAs that are functionally related.
The new miRNA RIP-chip system is designed to identify miRNAs bound to their mRNA targets, analyze the maturation mechanism of miRNAs, identify RISC components and analyze their functions.
Recent studies suggest that mRNAs encoding functionally related protein and their regulatory microRNAs bind to the common RNA binding proteins (RBPs), forming so-called RiboClusters.
MBL’s RIP-Assay Kit for micro RNA enables you to immunoprecpitate RiboClusters through RBP specific antibodies and isolate the RNAs and their regulatory micro RNAs associated with the RiboCluster (RIP Assay). You can thus isolate cluster specific mRNAs as well as miRNAs for subsequent analysis by microarray, sequencing or RT-PCR.

miRNA Assay Kit

miRNA Validated Antibodies

Click here to view RIP Assay Kits from MBL
Click here to view related antibodies from MBL 

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