Applications for immunohistochemistry

Next to the regular antibody products, Monosan can offer you Monosan Reagents. This product line contains a huge portfolio of detection systems for immunohistochemistry purposes like Monosan Plus (AP) Polymer Kits, Monosan Plus (HRP) Broad Spectrum Kits and Monosan (HRP) Broad Spectrum Kits. The Monosan Reagents detection kits are designed for the qualitative detection of antigens in fixed paraffin-embedded tissue sections, in frozen tissue sections, and in cytological samples. (The kits are developed for use in combination with mono- and polyclonal primary antibodies and sera obtained from mouse, rabbit, rat, and guinea pig.)

Beside the new detection systems MONOSAN® Reagents is offering comprehensive accessories for the Immunohistochemistry:

• Dilution buffers

Example: 
MONOSAN®Antibody Diluent is developed for dilution of primary antibodies. Antibodies diluted with Antibody Diluent are primarily used in immunohistochemistry with formalin-fixed paraffin-embedded tissue sections, but also with frozen, HOPE-fixed, and cytological samples as well as in immunoblot procedures.

• Chromogens and Substrates

Example: 
MONOSAN®Permanent AEC Kit is intended for immunohistochemical and in situ-hybridisation staining procedures with horse radish peroxidase (HRP). AEC (3-Amino-9-ethylcarbazol) leads to the formation of a red-brown precipitate at the location of the target antigen or target nucleic acid. The precipitate is insoluble in aqueous mounting media and can be observed by light microscopy.

• Histo Pens

• Pre-treatment solutions

Example: 
MONOSAN® FastEnzyme is a ready-to-use solution developed for enzymatic epitope retrieval on formalin-fixed tissue sections on slides. This procedure (sometimes called PIER,Protease Induced Epitope Retrieval) is used in immunohistochemical staining procedures and prior to in situ hybridisations.

• Blocking reagents

Example:
The blocking solution MONOSAN®Peroxide Block is intended for inhibition of endogenous peroxidase activity in tissue sections. It is primarily intended to be used in immunohistochemistry on formalin-fixed paraffin-embedded samples if using a detection system with horse radish peroxidase.

• Buffers

Example: 
Monosan® HIER EDTA Buffer pH 8.0 is a solution developed for heat induced epitope retrieval (HIER) in formalin-fixed paraffin-embedded tissue sections on slides. This procedure is primarily used in immunohistochemistry.

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