Monday, 17 October 2011

R-Phycoerythrin (R-PE) - (phycobiliprotein of red algae)


Phycoerythrin (R-PE) is a constituent part of the photosynthesis apparatus of red algae. It consists of a protein and 34 phycoerythrobilin fluorochromes. This fluorochrome/protein ratio is the main reason for the bright intensity of R-PE. However, the low degree of photostability limits its range of application. Its high molecular weight and tendency to agglomerate must be taken into consideration during the use of R-PE conjugates. Non-specific binding of the fluorochrome to cells is occasionally observed. R-PE-conjugated antibodies should not be stored at -20°C, as freezing damages the fluorochrome.

Flow Cytometry

R-PE can be measured on all standard commercial flow cytometers and sorters. Its high light intensity makes it the fluorochrome of choice in the detection of antigens expressed in low concentrations. The R-PE fluorochrome is also often used in multicolour analysis. However, the spectral characteristics of R-PE require compensation with FITC and fluorochromes in the long-wave emission range. For intracellular stainings the size of the fluorochrome should be weighed against its bright intensity. Under certain circumstances it may be advantageous to use Alexa Fluor® conjugates, or FITC conjugates for intracellular applications.

To view Caltag Laboratories range of R-PE fluorochromes click here.

To view the full range of Caltag Laboratories fluorochromes and their applications click here.

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