Friday, 16 September 2011

Immunohistochemistry


Immunohistochemistry (IHC) refers to the method of localizing specific antigens in cells or tissue sections based on the binding of labelled antibody to antigens. The antigen-antibody interaction could be visualized by a marker such as enzyme, radiolabel, fluorophore or colloidal metal. IHC makes it possible to visualize the distribution and localization of specific cellular components within a cell or tissue.

There are two methods for the immunohistochemical detection of antigens in tissue, the direct and indirect methods. The direct method, it is one-step staining with a labelled antibody reacting directly to antigen in tissue sections. This method is simple and rapid but has a lack of sensitivity. The indirect method requires the use of two antibodies. One is an unlabelled primary antibody which reacts with tissue antigen and the other is a labelled secondary antibody which reacts with this primary antibody. The indirect method is more sensitive due to several secondary antibody reactions with different antigenic sites on the primary antibody and allows for signal amplification. Abnova uses the indirect method to detect antigens in tissue.

Complete collection of IHC Validated Antibodies Include:
Angiogenesis | Apoptosis | Binding | Cell Ad/Junc/Cytoskel | Cell Cycle | Cytokine | Enzyme | Membrane | Metabolism | Neurobiology | Plasma/Serum | Stem Cell | Signal Transduction | Transcriptions | Ubiquitin | Others


1 comment:

  1. Hi friends,

    You have provided a very good site to knowing about Immunohistochemistry. These process of detecting antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues...

    Innate Immunity

    ReplyDelete

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